AdipoGen Life Sciences

anti-TIE-2 (human), mAb (tek2)

CHF 435.00
In stock
AG-20T-0102-C100100 µgCHF 435.00
More Information
Product Details
Synonyms Tyrosine Kinase with Ig and EGF Homology Domains-2; Angiopoietin-1 Receptor; Tie2; CD202b
Product Type Monoclonal Antibody
Properties
Clone tek2
Isotype Mouse IgG1
Immunogen/Antigen Recombinant human soluble extracellular domain of TIE-2.
Application

ELISA: (1-2μg/ml)
Flow Cytometry: (2-5μg/ml)
Immunohistochemistry: (frozen section)
Western Blot: (2-5μg/ml)
Optimal conditions must be determined individually for each application.

Crossreactivity Human
Specificity

Native human TIE-2 in ELISA experiments and on the surface of different human cell types.

Purity Protein G purified.
Purity Detail Protein G purified.
Formulation Lyophilized.
Reconstitution Centrifuge vial prior to opening. Reconstitute with sterile water to a concentration of 0.1-1.0mg/ml.
Isotype Negative Control

Mouse IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

TIE-2 acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. It has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. It is required for normal angiogenesis and heart development during embryogenesis and for post-natal hematopoiesis. After birth, it activates or inhibits angiogenesis, depending on the context. It inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1 and ultimately to the stimulation of sprouting angiogenesis.

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