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AdipoGen Life Sciences
anti-IL-1α (p18) (mouse), mAb (Teo-1)
170
CHF
CHF 170.00
In stock
AG-20B-0064-C100100 µgCHF 170.00
Figure 1: Mouse IL-1α (full-length p30 and cleaved p18 fragments) are detected by immunoblotting using anti-IL-1α (p18) (mouse), mAb (Teo-1) (Prod. No AG-20B-0064).
Method: IL-1α was analyzed by Western blot in cell extracts of bone marrow-derived dendritic cells (BMDCs) treated by LPS and the several inflammasome activators as indicated in the figure. Cell extracts were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-IL-1α (p18) (mouse), mAb (Teo-1) (1µg /ml). After addition of an anti-mouse secondary antibody coupled to HRP, proteins were visualized by a chemiluminescence detection system.
Method: IL-1α was analyzed by Western blot in cell extracts of bone marrow-derived dendritic cells (BMDCs) treated by LPS and the several inflammasome activators as indicated in the figure. Cell extracts were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-IL-1α (p18) (mouse), mAb (Teo-1) (1µg /ml). After addition of an anti-mouse secondary antibody coupled to HRP, proteins were visualized by a chemiluminescence detection system.
| Product Details | |
|---|---|
| Synonyms | Interleukin-1α IL-1alpha |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Teo-1 |
| Isotype | Mouse IgG |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse mature IL-1α. |
| Application |
Western Blot: (1μg/ml) ELISA |
| Crossreactivity | Mouse |
| Specificity | Recognizes mouse IL-1α p18 cleaved and full-length fragments. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The most prominent members of the interleukin-1 (IL-1) superfamily are IL-1α and IL-1β. They lack a signal peptide and are secreted by an unconventional, endoplasmic reticulum-Golgi-independent mechanism. IL-1α was reported to be more widely and constitutively expressed and has intracellular functions, but also acts locally in a membrane-bound form by activating IL-1R1. Additionally, passive release of IL-1α upon cell death can trigger a sterile inflammatory response to dying cells. The cleavage of IL-1α is not mediated by caspase-1 and is not required for binding to IL-1R1. Recently it has been observed that all activators of the inflammasome NLRP3/NALP3 induce the simultaneous secretion of IL-1α and IL-1β. Although most activators fully rely on the inflammasome for IL-1α secretion, some induce the processing and secretion of IL-1α in an inflammasome-independent manner.
Product References
- Formyl peptide receptor 1 signaling potentiates inflammatory brain injury: Z. Li, et al.; Sci. Transl. Med. 13, 605 (2021)
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