AdipoGen Life Sciences

anti-Notch1 (mouse), mAb (22E5) (Biotin)

CHF 350.00
In stock
AG-20B-0051B-C100100 µgCHF 350.00
More Information
Product Details
Synonyms Neurogenic Locus Notch Homolog Protein 1; Motch A; mT14; p300
Product Type Monoclonal Antibody
Properties
Clone 22E5
Isotype Rat IgG2aκ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse Notch1:Fc (AG-40B-0109).
Label/Conjugates Biotin
Application

Flow Cytometry: (1:500)

Immunofluorescence: (1:1000) (see Reference 1)

Crossreactivity Mouse
Specificity

Recognizes endogenous mouse Notch-1 receptor.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 0.5mg/ml
Formulation Liquid. In PBS containing 0.02% sodium azide.
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Notch signaling pathway regulates many different cell fate decisions in both vertebrate and invertebrate species. There are 5 canonical Notch ligands in mammals: Jagged-1, Jagged-2, DLL1, DLL3 and DLL4. These can bind to the four Notch receptors Notch 1-4. It is important for pattern formation during development such as neurogenesis, angiogenesis or myogenesis and regulates T cell development and stem cell maintenance. Notch signaling is also involved in cellular processes through-out adulthood. Signaling via Notch occurs between neighbouring cells and both the receptor and its ligands are transmembrane proteins.

Product References
  1. Dynamic Regulation of Notch 1 and Notch 2 Surface Expression during T Cell Development and Activation Revealed by Novel Monoclonal Antibodies: E. Fiorini, et al.; J. Immunol.183, 7212 (2009)
  2. Cutting edge: Thymic NK cells develop independently from T cell precursors: V.S. Ribeiro, et al.; J. Immunol. 185, 4993 (2010)
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