Cookie Policy: This site uses cookies to improve your experience. You can find out more about our use of cookies in our Privacy Policy. By continuing to browse this site you agree to our use of cookies.
AdipoGen Life Sciences
anti-Caspase-1 (p10) (mouse), mAb (Casper-2) (Biotin)
500
CHF
CHF 500.00
In stock
AG-20B-0044B-C100100 µgCHF 500.00
| Product Details | |
|---|---|
| Synonyms | Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Casper-2 |
| Isotype | Mouse IgG2a |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse caspase-1. |
| Label/Conjugates | Biotin |
| Application | Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation) |
| Crossreactivity | Mouse |
| Specificity | Recognizes endogenous full-length and activated (p10 fragment) mouse caspase-1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
Product References
- Measuring the inflammasome: O. Gross; Methods Mol. Biol. 844, 199 (2012)
- XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014)
- AMP-activated protein kinase suppresses urate crystal-induced inflammation and transduces colchicine effects in macrophages: Y. Wang, et al.; Ann. Rheum. Dis. 75, 286 (2016)
- Bile acids control inflammation and metabolic disorder through inhibition of NLRP3 inflammasome: C. Guo, et al.; Immunity 45, 802 (2016)
- Ablation of caspase-1 protects against TBI-induced pyroptosis in vitro and in vivo: W. Liu, et al.; J. Neuroinflamm. 15, 48 (2018)
- Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity: D. Boucher, et al.; J. Exp. Med. 215, 827 (2018)
- HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Diff. 10, 256 (2019)
Related Products
