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AdipoGen Life Sciences
anti-Caspase-1 (p10) (mouse), mAb (Casper-2)
480
CHF
CHF 480.00
In stock
AG-20B-0044-C100100 µgCHF 480.00
Figure 1: Mouse Caspase-1 (p10) is detected by immunoblotting using anti-Caspase-1 (p10) (mouse), mAb (Casper-2) (Prod. No AG-20B-0044). Method: Caspase-1 was analyzed by Western blot in supernatants of differentiated bone marrow-derived dendritic cells (BMDCs) from wild-type and caspase-1-/- mice activated or not by 5 μM Nigericin for 30 min (Prod. No. AG-CN2-0020). Supernatants (30μl) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-Caspase-1 (p10) (mouse), mAb (Casper-2) (1μg /ml). Proteins were visualized by a chemiluminescence detection system.
| Product Details | |
|---|---|
| Synonyms | Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Casper-2 |
| Isotype | Mouse IgG2a |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse caspase-1. |
| Application |
Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes endogenous full-length and activated (p10 fragment) mouse caspase-1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
Product References
- Measuring the inflammasome: O. Gross; Methods Mol. Biol. 844, 199 (2012)
- XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. 7, 1796 (2014)
- AMP-activated protein kinase suppresses urate crystal-induced inflammation and transduces colchicine effects in macrophages: Y. Wang, et al.; Ann. Rheum. Dis. 75, 286 (2016)
- Bile acids control inflammation and metabolic disorder through inhibition of NLRP3 inflammasome: C. Guo, et al.; Immunity 45, 802 (2016)
- IL-18 is critical for MAIT cell IFN- responses to Francisella 1 species in vitro but not in vivo: E. Jesteadt, et al.; Infect. Immun. 86, e00117 (2018)
- Interleukin-18 Is Critical for Mucosa-Associated Invariant T Cell Gamma Interferon Responses to Francisella Species In Vitro but Not In Vivo: E. Jesteadt, et al.; Infect. Immun. 86, e00117 (2018)
- Caspase-1 self-cleavage is an intrinsic mechanism to terminate inflammasome activity: D. Boucher, et al.; J. Exp. Med. 215, 827 (2018)
- Cholesterol Homeostatic Regulator SCAP-SREBP2 Integrates NLRP3 Inflammasome Activation and Cholesterol Biosynthetic Signaling in Macrophages: Ch. Guo, et al.; Immunity 49, 842 (2018)
- Repositioning of the β-Blocker Carvedilol as a Novel Autophagy Inducer That Inhibits the NLRP3 Inflammasome: WT. Wong, et al.; Front. Immunol. 9, 1920 (2018)
- HSP70 is a negative regulator of NLRP3 inflammasome activation: P. Martine, et al.; Cell Death Diff. 10, 256 (2019)
- Critical Role for the NLRP3 Inflammasome in Mediating IL-1β Production in Shigella sonnei-Infected Macrophages: LH. Li, et al. Front. Immunol. 11, 1115 (2020)
- Mechanism of miR-126 in hypoxia-reoxygenationinduced cardiomyocyte pyroptosis by regulating HMGB1 and NLRP3 inflammasome: L. Fei, et al.; Immunopharmacol. Immunotoxicol. 44, 500 (2022)
- CARD-only proteins regulate in vivo inflammasome responses and ameliorate gout: S. Devi, et al.; Cell Rep. 42, 112265 (2023)
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