AdipoGen Life Sciences

anti-Caspase-1 (p20) (mouse), mAb (Casper-1)

CHF 480.00
In stock
AG-20B-0042-C100100 µgCHF 480.00
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Product Details
Synonyms Interleukin-1β Convertase; IL-1BC; Interleukin-1β-converting Enzyme; ICE
Product Type Monoclonal Antibody
Properties
Clone Casper-1
Isotype Mouse IgG1
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse caspase-1.
Application

Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation)
Immunohistochemistry: (1:500; paraffin sections)
Immunoprecipitation: (1:200)

Crossreactivity Mouse
Rat
Specificity

Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1. Described to cross-react with full-length and activated (p20 fragment) of rat caspase-1 (Lit. #30 & 34).

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Mouse IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
Protocols Download PDF
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.

Product References
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