Additional Information
| Product Data | |
|---|---|
| Synonyms | Interleukin-1 β Convertase; IL-1BC; Interleukin-1 β-converting Enzyme; ICE |
| Properties | |
| Clone | Casper-1 |
| Isotype | Mouse IgG1 |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse caspase-1. |
| Application | Western Blot (see online protocol): (1μg/ml) (no need to precipitate the cell supernatant for the detection of caspase-1 (mouse) upon inflammasome activation) Immunohistochemistry: (1:500; paraffin sections) Immunoprecipitation: (1:200) |
| Crossreactivity | Mouse |
| Specificity | Recognizes endogenous full-length and activated (p20 fragment) mouse caspase-1. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Product Type | Monoclonal Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols |
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| MSDS |
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Product Description
Caspase-1 is the best-described inflammatory caspase. It processes the cytokines interleukin-1β (IL-1β) and IL-18 and induces pyroptotic cell death. Caspase-1 is activated by multiprotein complexes called Inflammasomes in response to numerous stimuli that are detected through distinct inflammasomes. NLRC4 responds to cytosolic flagellin, murine NLRP1b responds to anthrax lethal toxin, AIM2 responds to cytosolic DNA and NLRP3 responds to a variety of agonists including crystals.
Product References
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Mouse caspase-1 (p20) is detected by immunoblotting using anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (Prod. No. AG-20B-0042). Method: Caspase-1 was analyzed by Western blot in cell extracts and supernatants of differentiated bone marrow-derived dendritic cells (BMDCs) from wild-type, NLRP3-/- and caspase-1-/- mice activated or not by 5 μM Nigericin (Prod. No. AG-CN2-0020) for 30 min. Cell extracts and supernatants were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (1μg/ml). Proteins were visualized by a chemiluminescence detection system.
Immunohistochemical staining of endogenous mouse Caspase-1 in mouse spleen using anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (Prod. No. AG-20B-0042). Method: Mouse spleen tissues (paraffin sections) from Caspase-1 KO (left) or WT (right) mice were stained using anti-Caspase-1 (p20) (mouse), mAb (Casper-1) (Prod. No. AG-20B-0042) (1:500) by standard immunohistochemistry (antigen retrieval performed with sodium citrate).
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