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AdipoGen Life Sciences
anti-PIDD (mouse), mAb (Lise-1)
460
CHF
CHF 460.00
In stock
AG-20B-0038-C100100 µgCHF 460.00
Figure 1: Mouse PIDD is detected in primary MEF cells using anti-PIDD (mouse), mAb (Lise-1) (Prod. N°AG-20B-0038).
Method:: Cell extracts from mouse embryo fibroblasts (MEFs) either from WT (A-B) or PIDD KO cells (C-D) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-PIDD (mouse), mAb (Lise-1) (1µg/ml). Proteins were visualized by a chemiluminescence detection system.
Method:: Cell extracts from mouse embryo fibroblasts (MEFs) either from WT (A-B) or PIDD KO cells (C-D) were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with anti-PIDD (mouse), mAb (Lise-1) (1µg/ml). Proteins were visualized by a chemiluminescence detection system.
| Product Details | |
|---|---|
| Synonyms | p53-induced Protein with a Death Domain; Leucine-rich Repeat and Death Domain-containing Protein; Lrdd |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | Lise-1 |
| Isotype | Mouse IgG2bκ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Mouse PIDD (Death Domain). |
| Application |
Western Blot: (1:1'000) |
| Crossreactivity | Mouse |
| Specificity |
Recognizes mouse PIDD. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
PIDD promotes apoptosis downstream of the tumor suppressor as component of the DNA damage/stress response pathway that connects p53/TP53 to apoptosis. It associates with NEMO/IKBKG and RIP1 and enhances sumoylation and ubiquitination of NEMO/IKBKG which is important for activation of the transcription factor NF-κ-B. It associates with caspase-2 and RAIDD, and induces activation of caspase-2 which is an important regulator in the apoptotic pathways.
Product References
- PIDD orchestrates translesion DNA synthesis in response to UV irradiation: E. Logette, et al.; Cell Death Diff. 18, 1036 (2011)
- PIDDosome-SCAP crosstalk controls high-fructose-diet-dependent transition from simple steatosis to steatohepatitis: J.Y. Kim, et al.; Cell Metab. 34, (2022)
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