Additional Information
| Product Data | |
|---|---|
| Synonyms | NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein |
| Properties | |
| Clone | Cryo-2 |
| Isotype | Mouse IgG2b |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93). |
| Application | Western Blot (see online protocol): (1μg/ml) Immunocytochemistry: (see reference Nr. 6 and 10) Immunoprecipitation: (in RIPA buffer, see reference Nr. 8) Immunohistochemistry: (1:200, for a protocol see reference Nr. 9) |
| Crossreactivity | Human, Mouse |
| Specificity | Recognizes mouse and human NLRP3/NALP3. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Product Type | Monoclonal Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| Protocols | 
Download |
| MSDS |
Download |
Product Description
The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1β and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).
Product References
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Human and mouse NLRP3/NALP3 are detected in THP1 cells or mouse macrophages, respectively, using anti-NLRP3/NALP3, mAb (Cryo-2) (Prod. No. AG-20B-0014).
A) Cell extracts from the human cells THP1 (lane 1) or THP1 expressing shRNA-hNLRP3 (lane 2); B) Cell extracts from mouse macrophages, WT (lane 1) or NALP3/NLRP3 KO (lane 2), were separated by SDS-PAGE, transferred to nitrocellulose and incubated with anti-NLRP3/NALP3, mAb (Cryo-2) (1μg/ml). Proteins were visualized by a chemiluminescence detection system.
A) Cell extracts from the human cells THP1 (lane 1) or THP1 expressing shRNA-hNLRP3 (lane 2); B) Cell extracts from mouse macrophages, WT (lane 1) or NALP3/NLRP3 KO (lane 2), were separated by SDS-PAGE, transferred to nitrocellulose and incubated with anti-NLRP3/NALP3, mAb (Cryo-2) (1μg/ml). Proteins were visualized by a chemiluminescence detection system.
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