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anti-NLRP3/NALP3, mAb (Cryo-2)

AG-20B-0014-C100 100 µg INQ
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Product Data
Synonyms NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein
Properties
Clone Cryo-2
Isotype Mouse IgG2b
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).
Application Western Blot (see online protocol): (1μg/ml)
Immunocytochemistry: (see reference Nr. 6 and 10) Immunoprecipitation: (in RIPA buffer, see reference Nr. 8) Immunohistochemistry: (1:200, for a protocol see reference Nr. 9)
Crossreactivity Human, Mouse
Specificity Recognizes mouse and human NLRP3/NALP3.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Product Type Monoclonal Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
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MSDS Download Document Download
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Product Description

The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1β and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).

Product References

  1. Thioredoxin-interacting protein links oxidative stress to inflammasome activation: R. Zhou, et al.; Nature Immunology 11, 136 (2010)
  2. The NLRP3 inflammasome promotes renal inflammation and contributes to CKD: A. Vilaysane, et al.; J. Am. Soc. Nephrol. 21, 1732 (2010)
  3. A role for mitochondria in NLRP3 inflammasome activation: R. Zhou, et al.; Nature 469, 221 (2011)
  4. Differential expression of NLRP3 among hematopoietic cells: G. Guarda, et al.; J. Immunol. 186, 2529 (2011)
  5. Mechanism of Impaired NLRP3 Inflammasome Priming by Monophosphoryl Lipid A: C.A. Embry, et al.; Sci. Signal. 4, ra28 (2011)
  6. Measles Virus V Protein Inhibits NLRP3 Inflammasome-Mediated Interleukin-1 Secretion: N. Komune, et al.; J. Virol. 85, 13019 (2011)
  7. Novel role of PKR in inflammasome activation and HMGB1 release: B. Lu, et al.; Nature 488, 670 (2012)
  8. Deubiquitination of NLRP3 by BRCC3 Critically Regulates Inflammasome Activity: B.F. Py, et al.; Mol. Cell 49, 331 (2012)
  9. NLRP3 is activated in Alzheimer's disease and contributes to pathology in APP/PS1 mice: M.T. Heneka, et al.; Nature 493, 67 (2012)
  10. Activation of autophagy by inflammatory signals limits IL-1b production by targeting ubiquitinated inflammasomes for destruction: C.-S. Shi, et al.; Nat. Immunol. 13, 255 (2012)
  11. Inflammasome-independent NLRP3 augments TGF-β signaling in kidney epithelium: W. Wang, et al.; J. Immunol. 190, 1239 (2013)
  12. Omega-3 Fatty Acids Prevent Inflammation and Metabolic Disorder through Inhibition of NLRP3 Inflammasome Activation: Y. Yan, et al.; Immunity 38, 1154 (2013)
  13. LRRFIP2 negatively regulates NLRP3 inflammasome activation in macrophages by promoting Flightless-I-mediated caspase-1 inhibition: J. Jin, et al.; Nat. Commun. 4, 2075 (2013)
  14. Proapoptotic Chemotherapeutic Drugs Induce Noncanonical Processing and Release of IL-1β via Caspase-8 in Dendritic Cells: C. Antonopoulus, et al. J. Immunol. in press (2013)
  15. Constitutively activated NLRP3 inflammasome causes inflammation and abnormal skeletal development in mice: S.L. Bonar, et al.; PLoS One 7, e35979 (2012)
  16. FADD and caspase-8 mediate priming and activation of the canonical and noncanonical Nlrp3 inflammasomes: P. Gurung, et al.; J. Immunol. 192, 1835 (2014)
  17. XIAP Restricts TNF- and RIP3-Dependent Cell Death and Inflammasome Activation: M. Yabal, et al.; Cell Rep. (Epub ahead of print) (2014)
  18. The adaptor ASC has extracellular and 'prionoid' activities that propagate inflammation: B.S. Franklin, et al.; Nat. Immunol. (Epub ahead of print) (2014)
  19. The NLRP3 inflammasome is released as a particulate danger signal that amplifies the inflammatory response: A. Baroja-Mazo, et al.; Nat. Immunol. (Epub ahead of print) (2014)
Human and mouse NLRP3/NALP3 are detected in THP1 cells or mouse macrophages, respectively, using anti-NLRP3/NALP3, mAb (Cryo-2) (Prod. No. AG-20B-0014).<br /><b>Method:</b>A) Cell extracts from the human cells THP1 (lane 1) or THP1 expressing shRNA-hNLRP3 (lane 2); B) Cell extracts from mouse macrophages, WT (lane 1) or NALP3/NLRP3 KO (lane 2), were separated by SDS-PAGE, transferred to nitrocellulose and incubated with anti-NLRP3/NALP3, mAb (Cryo-2) (1&mu;g/ml). Proteins were visualized by a chemiluminescence detection system.
Human and mouse NLRP3/NALP3 are detected in THP1 cells or mouse macrophages, respectively, using anti-NLRP3/NALP3, mAb (Cryo-2) (Prod. No. AG-20B-0014).
Method:A) Cell extracts from the human cells THP1 (lane 1) or THP1 expressing shRNA-hNLRP3 (lane 2); B) Cell extracts from mouse macrophages, WT (lane 1) or NALP3/NLRP3 KO (lane 2), were separated by SDS-PAGE, transferred to nitrocellulose and incubated with anti-NLRP3/NALP3, mAb (Cryo-2) (1μg/ml). Proteins were visualized by a chemiluminescence detection system.
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