|Synonyms||NACHT-, LRR- and PYD-containing Protein 3; NLRP3; Cryopyrin; Cold Autoinflammatory Syndrome 1 Protein|
|Source/Host||Purified from concentrated hybridoma tissue culture supernatant.|
|Immunogen/Antigen||Recombinant mouse NLRP3/NALP3 (pyrin domain/aa 1-93).|
|Application||Western Blot (see online protocol): (1μg/ml)
Immunocytochemistry: (see J. Virol. 85, 13019 (2011) and Nat. Immunol. 13, 255 (2012))
Immunoprecipitation: (in RIPA buffer, see Mol. Cell 49, 331 (2012))
Immunohistochemistry: (1:200, for a protocol see Nature 493, 67 (2012))
ChIP assay: 2µg/DNA of 10E6 cells (see Nat. Immunol. 16, 859 (2015))
|Specificity||Recognizes mouse and human NLRP3/NALP3.|
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.|
|Product Type||Monoclonal Antibody|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1β and IL-18. Members of the Nod-like receptor family, including NLRP3/NALP3, are critical components of the inflammasome that link danger-signals to caspase-1 activation. Defects in NLRP3 are the cause of familial cold autoinflammatory syndrome type 1 (FCAS1), Muckle-Wells syndrome (MWS) and of chronic infantile neurologic cutaneous and articular syndrome (CINCA).
Mouse NLRP3 is detected in mouse macrophages using the monoclonal antibody to NLRP3 (Cryo-2) (Prod. No. AG-20B-0014). Method: Cell extracts from mouse macrophages (BMDMs) WT +/+ (lane 1), NLRP3+/- (lane 2) or NLRP3 -/- (lane 3), with or without treatment with LPS (50ng/ml) for 3h, were separated by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the mAb to NLRP3 (Cryo-2) (1µg/ml). Proteins are visualized by a chemiluminescence detection system. Picture courtesy of Dr. Olaf Gross, Munich University, Germany.
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