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AdipoGen Life Sciences
anti-LAG-3 (human), mAb (17B4) (ATTO 647N)
490
CHF
CHF 490.00
In stock
AG-20B-0012TS-T100100 testsCHF 490.00
Figure 1: Detection of endogenous human LAG-3 by FACS analysis using anti-LAG-3 (human), mAb (17B4) (ATTO 647) (Prod. No. AG-20B-0012TS).
Human PBMC were stimulated (B) or not (A) with 1μg/ml of superantigen SEB. After 2 days PBMC were stained with 10μg/ml (1μg/0.5x106 cells) of anti-LAG-3 (human), mAb (17B4) (ATTO 647) (Prod. No. AG-20B-0012TS) and analyzed by flow cytometry.
Human PBMC were stimulated (B) or not (A) with 1μg/ml of superantigen SEB. After 2 days PBMC were stained with 10μg/ml (1μg/0.5x106 cells) of anti-LAG-3 (human), mAb (17B4) (ATTO 647) (Prod. No. AG-20B-0012TS) and analyzed by flow cytometry.
| Product Details | |
|---|---|
| Synonyms | Lymphocyte Activation Gene-3; FDC Protein; CD223 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 17B4 |
| Isotype | Mouse IgG1κ |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Synthetic peptide corresponding to 30 aa in the N-terminus of human LAG-3. |
| Label/Conjugates | ATTO 647N |
| Application |
Flow Cytometry: (1:100) |
| Crossreactivity | Human |
| Specificity |
Recognizes human LAG-3. |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 0.02% sodium azide. |
| Isotype Negative Control | |
| Other Product Data |
New ATTO-fluorescent antibodies show increased photostability, outstanding brightness and intense signals. ATTO dyes are thermally stable, resistant to environmental changes and show no significant isomerization. |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | +4°C |
| Handling Advice |
Do not freeze. Protect from light. |
| Use/Stability |
Keep conjugated formats at +4°C. Stable for at least 1 year after receipt when stored at +4°C. |
| Documents | |
| Protocols |
 Download PDF |
| MSDS |
Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Lymphocyte activation gene 3 (LAG-3; CD223) plays an important role in negatively regulating T cell proliferation, function and homeostasis. It is required for maximal natural and induced regulatory T cell (Treg) function. LAG-3 is closely related to the T cell co-receptor CD4 and binds to MHC class II molecules but with a significantly higher affinity than CD4.
Product References
- Characterization of the lymphocyte activation gene 3-encoded protein. A new ligand for human leukocyte antigen class II antigens: E. Baixeras, et al.; J. Exp. Med.176, 327 (1992)
- Cellular expression and tissue distribution of the human LAG-3-encoded protein, an MHC class II ligand: B. Huard, et al.; Immunogenetics 39, 213 (1994)
- Characterization of the major histocompatibility complex class II binding site on LAG-3 protein: B. Huard, et al.; PNAS 94, 5744 (1997)
- CD3/TCR complex-associated lymphocyte activation gene-3 molecules inhibit CD3/TCR signaling: S. Hannier, et al.; J. Immunol. 161, 4058 (1998)
- T Lymphocytes infiltrating various tumour types express the MHC class II ligand lymphocyte activation gene-3 (LAG-3): role of LAG-3/MHC class II interactions in cell-cell contacts: C.E. Demeure, et al.; Eur. J. Cancer 37, 1709 (2001)
- Immunological mechanisms elicited at the tumour site by lymphocyte activation gene-3 (LAG-3) versus IL-12: sharing a common Th1 anti-tumour immune pathway: E.D. Carlo, et al.; J. Pathol. 205, 82 (2005)
- The negative regulatory function of the lymphocyte-activation gene-3 co-receptor (CD223) on human T cells: L. Macon-Lemaitre and F. Triebel; Immunology 115,170 (2005)
- A soluble lymphocyte activation gene-3 (sLAG-3) protein as a prognostic factor in human breast cancer expressing estrogen or progesterone receptors: F. Triebel, et al.; Cancer Lett. 235, 147 (2006)
- Human dendritic cells acquire a semimature phenotype and lymph node homing potential through interaction with CD4+CD25+ regulatory T cells: J. Bayry, et al.; J. Immunol. 178, 4184 (2007)
- Human lymphocyte activation gene-3 molecules expressed by activated T cells deliver costimulation signal for dendritic cell activation: C. Casati, et al.; J. Immunol. 180, 3782 (2008)
- Targeting LAG-3 and PD-1 to Enhance T Cell Activation by Antigen-Presenting Cells: F.S. Lichtenegger, et al.; Front. Immunol. 9, 385 (2018)
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