Call +41-61-926-60-40 Fax +41-61-926-60-49

anti-ZBP1, mAb (Zippy-1)

AG-20B-0010-C100 100 µg INQ
Actions
Select size:

* Required Fields

Amount:
Add to Cart
Call +41-61-926-60-40
Fax +41-61-926-60-49
Contact Us!

Additional Information

Product Data
Synonyms Z-DNA-binding Protein 1; Tumor Stroma and Activated Macrophage Protein DLM-1; DNA-dependent Activator of IFN-regulatory Factors; DAI
Properties
Clone Zippy-1
Isotype Mouse IgG2a
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse ZBP1 (aa 1-411).
Application Immunocytochemistry: (1:500) (see reference 1)
Immunoprecipitation: (1:200)
Western Blot: (1:1’000)
Crossreactivity Human, Mouse
Specificity Recognizes human and mouse ZBP1.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Product Type Monoclonal Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download Document Download

Product Description

ZBP1 is an interferon inducible protein that binds to cytoplasmic DNA and plays a role in host defense against tumors and pathogens. ZBP1 is highly expressed in lymphatic tissues including lymph node, leukocytes, tonsil, bone marrow and spleen. ZBP1 is an innate immune sensor of Influenza A virus (IAV). ZBP1 regulates NLRP3 (nucleotide and oligomerization domain, leucine-rich repeat-containing protein family, pyrin domain containing 3) inflammasome activation as well as induction of different types of cell death (apoptosis, necroptosis and pyroptosis) during IAV infection.

Product References

  1. ZBP1/DAI is an innate sensor of influenza virus triggering the NLRP3 inflammasome and programmed cell death pathways: T. Kuriakose, et al.; Sci. Immunol. 1, aag2045 (2016)
  2. DAI senses Influenza A virus genomic RNA and activates RIPK3-dependent cell death: R.J. Thapa, et al.; Cell Host & Microbe 20, 674 (2016)
  3. RIPK1 counteracts ZBP1-mediated necroptosis to inhibit inflammation: J. Lin, et al.; Nature 540, 124 (2016)
  4. RIPK1 inhibits ZBP1-driven necroptosis during development: K. Newton, et al.; Nature 540, 129 (2016)
Western Blot analysis of mouse ZBP1 in L929 cells by using anti-ZBP1, mAb (Zippy-1) (Prod. No. AG-20B-0010).<br />
Cell extracts from L929 cell either unstimulated (lane 1) or stimulated for 24h with poly(dA.dT) poly(dT.dA) at 3&mu;g/ml (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the anti-ZBP1, mAb (Zippy-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. The multiple bands represent isoforms or degradation products of ZBP1 since they are down regulated upon ZBP1 siRNA treatment (data not shown).
Western Blot analysis of mouse ZBP1 in L929 cells by using anti-ZBP1, mAb (Zippy-1) (Prod. No. AG-20B-0010).
Cell extracts from L929 cell either unstimulated (lane 1) or stimulated for 24h with poly(dA.dT) poly(dT.dA) at 3μg/ml (lane 2) were resolved by SDS-PAGE under reducing conditions, transferred to nitrocellulose and incubated with the anti-ZBP1, mAb (Zippy-1) at a 1:1000 dilution for 1 hour. Proteins were visualized using a peroxidase-conjugated polyclonal antibody to mouse IgG and a chemiluminescence detection system. The multiple bands represent isoforms or degradation products of ZBP1 since they are down regulated upon ZBP1 siRNA treatment (data not shown).
No reviews for this product yet.