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AdipoGen Life Sciences
anti-NS3 (HCV), mAb (1B6)
400
CHF
CHF 400.00
In stock
AG-20B-0001-C100100 µgCHF 400.00
| Product Details | |
|---|---|
| Synonyms | Nonstructural Protein 3 |
| Product Type | Monoclonal Antibody |
| Properties | |
| Clone | 1B6 |
| Isotype | Mouse IgG1 |
| Source/Host | Purified from concentrated hybridoma tissue culture supernatant. |
| Immunogen/Antigen | Recombinant hepatitis C virus (HCV) NS3 serine protease domain. |
| Application |
Immunocytochemistry: (paraformaldehyde fixation) (1:50) |
| Crossreactivity | HCV |
| Specificity |
Recognizes HCV NS3 (genotype 1a and 1b) (epitope mapped to NS3 aa 160-193), recognizes NS3 alone or the NS3-4A complex. Does not cross-react with HCV NS3 (genotype 2a). |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Isotype Negative Control | |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The NS3 serine protease, in association with NS4A, is responsible for the cleavages of the Hepatitis C virus protease complexes NS3-NS4A, NS4A-NS4B, NS4B-NS5A and NS5A-NS5B. NS5B is a RNA-dependent RNA polymerase that plays an essential role in the virus replication. Hepatitis C virus protease NS3-NS4A colocalizes with IPS-1/VISA/MAVS/Cardif in the mitochondrial membrane and has been shown to cleave the TLR3 adapter TRIF and Cardif to eliminate the antiviral signaling pathways. NS3-NS4A represents an important target for anti-HCV drugs.
Product References
- Subcellular localization, stability, and trans-cleavage competence of the hepatitis C virus NS3-NS4A complex expressed in tetracycline-regulated cell lines: B. Wolk, et al.; J. Virol. 74, 2293 (2000)
- Protein-protein interactions between hepatitis C virus nonstructural proteins: Dimitrova, et al.; J. Virol. 77, 5401 (2003)
- Identification of the hepatitis C virus RNA replication complex in Huh-7 cells harboring subgenomic replicons: R. Gosert, et al.; J. Virol. 77, 5487 (2003)
- Insertion of green fluorescent protein into nonstructural protein 5A allows direct visualization of functional hepatitis C virus replication complexes: D. Moradpour, et al.; J. Virol. 78, 7400 (2004)
- Cardif is an adaptor protein in the RIG-I antiviral pathway and is targeted by hepatitis C virus: E. Meylan, et al.; Nature 437, 1167 (2005)
- Hepatitis C virus RNA replication is regulated by host geranylgeranylation and fatty acids: S.B. Kapadia and F.V. Chisari; PNAS 102, 2561 (2005)
- Intracellular Delivery of HCV NS3p gene using vectored particles: M.A. Nobre Lemos, et al.; J. Biotech. 274, 33 (2018)
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