Additional Information
| Product Data | |
|---|---|
| Synonyms | Indoleamine 2,3-dioxygenase; INDO |
| Properties | |
| Clone | ID 177 |
| Isotype | Mouse IgG1κ |
| Immunogen/Antigen | Recombinant human IDO. |
| Application | ELISA: (direct or indirect: 1:2'000-1:10'000) Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application. |
| Crossreactivity | Human |
| Specificity | Recognizes human IDO. Detects a band of ~45kDa by Western blot. Other species not tested. |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Product Type | Monoclonal Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Documents | |
| MSDS | 
Download |
Product Description
IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-γ induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Western blot analysis using anti-IDO (human), mAb (ID 177) (Prod. No. AG-20A-0035) at 1:2'000 dilution.
1: Recombinant human IDO (His-tagged).
2: PHA-treated human peripheral blood lymphocyte lysate.
3: Con A-treated human peripheral blood lymphocyte lysate.
4: Human peripheral blood lymphocyte lysate.
1: Recombinant human IDO (His-tagged).
2: PHA-treated human peripheral blood lymphocyte lysate.
3: Con A-treated human peripheral blood lymphocyte lysate.
4: Human peripheral blood lymphocyte lysate.
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