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anti-IDO (human), mAb (ID 177)

AG-20A-0035-C050 50 µg $170.00
AG-20A-0035-C100 100 µg $240.00
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Additional Information

Product Data
Synonyms Indoleamine 2,3-dioxygenase; INDO
Properties
Clone ID 177
Isotype Mouse IgG1κ
Immunogen/Antigen Recombinant human IDO.
Application ELISA: (direct or indirect: 1:2'000-1:10'000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Optimal conditions should be determined individually for each application.
Crossreactivity Human
Specificity Recognizes human IDO. Detects a band of ~45kDa by Western blot. Other species not tested.
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Product Type Monoclonal Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Documents
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Product Description

IDO catalyzes the first and rate-limiting step in the main pathway of human tryptophan catabolism, the kynurenine pathway. Proinflammatory mediators, such as endotoxin and IFN-γ induce the expression of IDO in several tissues. IDO-dependent suppression of T-cell responses might function as natural immunoregulatory mechanism. Physiological IDO activity has been implicated in T-cell tolerance to tumors, dysfunctional selftolerance in non-obese diabetic (NOD) mice, and as a protective negative regulator in autoimmune disorders.
Western blot analysis using anti-IDO (human), mAb (ID 177) (Prod. No. AG-20A-0035) at 1:2'000 dilution.<br />
1: Recombinant human IDO (His-tagged).<br />
2: PHA-treated human peripheral blood lymphocyte lysate.<br />
3: Con A-treated human peripheral blood lymphocyte lysate.<br />
4: Human peripheral blood lymphocyte lysate.
Western blot analysis using anti-IDO (human), mAb (ID 177) (Prod. No. AG-20A-0035) at 1:2'000 dilution.
1: Recombinant human IDO (His-tagged).
2: PHA-treated human peripheral blood lymphocyte lysate.
3: Con A-treated human peripheral blood lymphocyte lysate.
4: Human peripheral blood lymphocyte lysate.
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