AdipoGen Life Sciences

anti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Biotin)

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AG-20A-0034B-C05050 µgCHF 350.00

Figure 1: Immunohistochemical staining of Nampt using anti-Nampt (Visfatin/PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B) in formalin-fixed and paraffin-embedded (FFPE) human colon tissue (15µg/ml).

Figure 2: Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Prod. No. AG-20A-0034B) in human kidney tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed and paraffin-embedded (FFPE) human kidney showing cytoplasmic staining (200x, brown color).

Figure 3: Immunofluorescent confocal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B). Nampt (Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).

Figure 4: Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034B) at 1:2000 dilution.
1. Human Nampt (Visfatin/PBEF) (His-tagged).
2. Human Nampt (Visfatin/PBEF) (FLAG^®-tagged).
3. Mouse Nampt (Visfatin/PBEF) (His-tagged).
4. Rat Nampt (Visfatin/PBEF) (His-tagged).
5. Human Nampt (Visfatin/PBEF) (His-tagged).
6. LPS-treated human peripheral blood leukocyte lysate.

Figure 5: Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) (Prod. No. AG-20A-0034B) at 1:2000 dilution.
1. IBMX. + DEX. - treated 3T3-L1 cell lysate.
2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.

Specifications / Handling

More Information
Product Details
Synonyms	Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Product Type	Monoclonal Antibody
Properties
Clone	OMNI379
Isotype	Mouse IgG2aκ
Immunogen/Antigen	Recombinant human Nampt (visfatin/PBEF).
Label/Conjugates	Biotin
Application	ELISA: (direct or indirect: 1:2'000-1:10'000) Immunocytochemistry: (1:500-1:1’000) Immunohistochemistry: (paraffin sections, 1:100-1:500) Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application.
Crossreactivity	Human Mouse Rat
Specificity	Recognizes human, mouse and rat Nampt.
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Isotype Negative Control	Mouse IgG2a Isotype Control (Biotin)
RRID	AB_2490118
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Scientific Background Information

Product Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Product-specific References

Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett. 580, 4105 (2006)
Molecular Characteristics of Serum Visfatin and Differential Detection by Immunoassays: A. Korner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007)
Nampt/PBEF/Visfatin Regulates Insulin Secretion in beta Cells as a Systemic NAD Biosynthetic Enzyme: J.R. Revollo, et al.; Cell. Metab. 6, 363 (2007)
Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes: A. Garten, et al.; BBRC 391, 376 (2010)
Intracellular nicotinamide phosphoribosyltransferase protects against hepatocyte apoptosis and is down-regulated in nonalcoholic fatty liver disease: T.B. Dahl, et al.; J. Clin. Endocrinol. Metab. 95, 3039 (2010) Supplement Information
Expression and regulation of nampt in human islets: K. Kover, et al.; PLoS One 8, e58767 (2013)
Extracellular nicotinamide phosphoribosyltransferase (NAMPT) promotes M2 macrophage polarization in chronic lymphocytic leukemia: V. Audrito, et al.; Blood 125, 111 (2015)
SIRT1-Mediated eNAMPT Secretion from Adipose Tissue Regulates Hypothalamic NAD+ and Function in Mice: M.J. Yoon, et al.; Cell Metab. 21, 706 (2015)
Nicotinamide phosphoribosyltransferase production in human spermatozoa is influenced by maturation stage: S. Riammer, et al.; Andrology 4, 1045 (2016)
Synthesis and degradation of adenosine 50-tetraphosphate by nicotinamide and nicotinate phosphoribosyltransferases: A. Amici, et al.; Cell Chem. Biol. 24, 553 (2017)
Nicotinamide phosphoribosyltransferase expression and clinical outcome of resected stage I/II pancreatic ductal adenocarcinoma: K. Davis, et al.; PLoS One 14, e0213576 (2019)
A nicotinamide phosphoribosyltransferase-GAPDH interaction sustains the stress-induced NMN/NAD+ salvage pathway in the nucleus: A.A. Grolla, et al.; J. Biol. Chem. 295, 3635 (2020)

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