Additional Information
| Product Data | |
|---|---|
| Synonyms | Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase |
| Properties | |
| Clone | OMNI379 |
| Isotype | Mouse IgG2aκ |
| Immunogen/Antigen | Recombinant human Nampt (visfatin/PBEF). |
| Application | ELISA: (direct or indirect: 1:2'000-1:10'000) Flow Cytometry: (1:1'000) Immunocytochemistry: (1:500-1:1’000) Immunohistochemistry: (paraffin sections (1:500-1:1’000)) Immunoprecipitation: (1:500-1:1’000) Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application. |
| Crossreactivity | Human, Mouse, Rat |
| Specificity | Recognizes human, mouse and rat Nampt. |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Product Type | Monoclonal Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Documents | |
| MSDS | 
Download |
Product Description
Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.
Product References
|
Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Prod. No. AG-20A-0034) in human tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney showing cytoplasmic staining (200x, brown color).
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) (Prod. No. AG-20A-0034) at 1: 2,000 dilution.
1. IBMX. + DEX. - treated 3T3-L1 cell lysate.
2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
1. IBMX. + DEX. - treated 3T3-L1 cell lysate.
2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034) at 1: 2,000 dilution.
1. Human Nampt(Visfatin/PBEF) (His-tagged).
2. Human Nampt(Visfatin/PBEF) (FLAG®-tagged).
3. Mouse Nampt(Visfatin/PBEF) (His-tagged).
4. Rat Nampt(Visfatin/PBEF) (His-tagged).
5. Human Nampt(Visfatin/PBEF) (His-tagged).
6. LPS-treated human peripheral blood leukocyte lysate.
1. Human Nampt(Visfatin/PBEF) (His-tagged).
2. Human Nampt(Visfatin/PBEF) (FLAG®-tagged).
3. Mouse Nampt(Visfatin/PBEF) (His-tagged).
4. Rat Nampt(Visfatin/PBEF) (His-tagged).
5. Human Nampt(Visfatin/PBEF) (His-tagged).
6. LPS-treated human peripheral blood leukocyte lysate.
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
No reviews for this product yet.