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anti-Nampt (Visfatin/PBEF), mAb (OMNI379)

AG-20A-0034-C050 50 µg $195.00
AG-20A-0034-C100 100 µg $290.00
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Additional Information

Product Data
Synonyms Pre-B Cell Colony Enhancing Factor 1; PBEF1; Nicotinamide Phosphoribosyltransferase
Properties
Clone OMNI379
Isotype Mouse IgG2aκ
Immunogen/Antigen Recombinant human Nampt (visfatin/PBEF).
Application ELISA: (direct or indirect: 1:2'000-1:10'000)
Flow Cytometry: (1:1'000)
Immunocytochemistry: (1:500-1:1’000)
Immunohistochemistry: (paraffin sections (1:500-1:1’000))
Immunoprecipitation: (1:500-1:1’000)
Western blot: (1:2'000-1:5'000 using ECL. Suggested blocking and dilution buffer is PBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Optimal conditions should be determined individually for each application.
Crossreactivity Human, Mouse, Rat
Specificity Recognizes human, mouse and rat Nampt.
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Product Type Monoclonal Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Documents
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Product Description

Nicotinamide phosphoribosyltransferase (Nampt; pre-B cell colony-enhancing factor; PBEF; Visfatin) is an 52kDa adipokine secreted by adipose tissue and involved in the biosynthesis of nicotinamide adenine dinucleotide (NAD+). Two forms of Nampt exist, an intracellular form (iNampt) and an extracellular form (eNampt). While the function of iNampt as an essential and rate-limiting NAD+ biosynthetic enzyme is well established, the physiological role of eNampt is still a matter of debate. Nampt has various functions, including the promotion of vascular smooth muscle cell maturation and inhibition of neutrophil apoptosis. It activates insulin receptor and has insulin-mimetic effects, lowering blood glucose and improving insulin sensitivity. The protein is highly expressed in visceral fat and serum levels of the protein correlate with obesity.

Product References

  1. Hypoxic induction of human visfatin gene is directly mediated by hypoxia-inducible factor-1: S.K. Bae, et al.; FEBS Lett. 580, 4105 (2006)
  2. Molecular Characteristics of Serum Visfatin and Differential Detection by Immunoassays: A. Korner, et al.; J. Clin. Endocrinol. Metab. 92, 4783 (2007)
  3. Nampt/PBEF/Visfatin Regulates Insulin Secretion in beta Cells as a Systemic NAD Biosynthetic Enzyme: J.R. Revollo, et al.; Cell. Metab. 6, 363 (2007)
  4. Nicotinamide phosphoribosyltransferase (NAMPT/PBEF/visfatin) is constitutively released from human hepatocytes: A. Garten, et al.; BBRC 391, 376 (2010)
  5. Intracellular nicotinamide phosphoribosyltransferase protects against hepatocyte apoptosis and is down-regulated in nonalcoholic fatty liver disease: T.B. Dahl, et al.; J. Clin. Endocrinol. Metab. 95, 3039 (2010) Supplement Information
  6. Expression and regulation of nampt in human islets: K. Kover, et al.; PLoS One 8, e58767 (2013)
  7. Extracellular nicotinamide phosphoribosyltransferase (NAMPT) promotes M2 macrophage polarization in chronic lymphocytic leukemia: V. Audrito, et al.; Blood (Epub ahead of print) (2014)
Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Prod. No. AG-20A-0034) in human tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney showing cytoplasmic staining (200x, brown color).
Immunohistochemical staining of Nampt using anti-Nampt (OMNI379) (Prod. No. AG-20A-0034) in human tissue (1:500 dilution). Immunoperoxidase staining of formalin-fixed, paraffin-embedded human kidney showing cytoplasmic staining (200x, brown color).
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) (Prod. No. AG-20A-0034) at 1: 2,000 dilution.<br />
1. IBMX. + DEX. - treated 3T3-L1 cell lysate.<br />
2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.<br />
Western blot analysis of 3T3-L1 cell lysate using anti-Nampt (Visfatin/PBEF) mAb (OMNI379) (Prod. No. AG-20A-0034) at 1: 2,000 dilution.
1. IBMX. + DEX. - treated 3T3-L1 cell lysate.
2. IBMX. + DEX. + Insulin - treated 3T3-L1 cell lysate.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034) at 1: 2,000 dilution.<br />
1. Human Nampt(Visfatin/PBEF) (His-tagged).<br />
2. Human Nampt(Visfatin/PBEF) (FLAG<sup>®</sup>-tagged).<br />
3. Mouse Nampt(Visfatin/PBEF) (His-tagged).<br />
4. Rat Nampt(Visfatin/PBEF) (His-tagged).<br />
5. Human Nampt(Visfatin/PBEF) (His-tagged).<br />
6. LPS-treated human peripheral blood leukocyte lysate.
Western blot analysis using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034) at 1: 2,000 dilution.
1. Human Nampt(Visfatin/PBEF) (His-tagged).
2. Human Nampt(Visfatin/PBEF) (FLAG®-tagged).
3. Mouse Nampt(Visfatin/PBEF) (His-tagged).
4. Rat Nampt(Visfatin/PBEF) (His-tagged).
5. Human Nampt(Visfatin/PBEF) (His-tagged).
6. LPS-treated human peripheral blood leukocyte lysate.
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Immunofluorescent confoacal imaging of HEK293 cells using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034). Nampt(Visfatin/PBEF) (green staining) is localized in the cytoplasm and nucleus (400x magnification). Nuclei are stained with Hoechst (blue staining).
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
Immunoprecipitation of recombinant human Nampt (Visfatin/PBEF) proteins using anti-Nampt (Visfatin-PBEF), mAb (OMNI379) (Prod. No. AG-20A-0034). Recombinant human Nampt (Visfatin/PBEF) proteins at different concentrations were precipitated. The precipitated proteins were separated by SDS-PAGE, electroblotted, and visualized by western blot with anti-human Nampt(Visfatin/PBEF) pAb.
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