Additional Information
| Product Data | |
|---|---|
| Synonyms | ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa |
| Properties | |
| Clone | HADI 773 |
| Isotype | Mouse IgG1κ |
| Immunogen/Antigen | Recombinant human adiponectin. |
| Application | ELISA: (direct and indirect: 1:5'000-1:10'000). Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature). Western blot: (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature). Optimal conditions must be determined individually for each application. |
| Crossreactivity | Human |
| Specificity | Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. Cross-reactivity with other species not tested. |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Product Type | Monoclonal Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Documents | |
| MSDS | 
Download |
Product Description
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
Product References
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Western Blot analysis of human plasma adiponectin using anti-Adiponectin (human), mAb (HADI 773) (Prod. No. AG-20A-0001) at 0.2μg/ml.
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