|Synonyms||ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa|
|Immunogen/Antigen||Recombinant human adiponectin.|
|Application||ELISA: (direct and indirect: 1:5'000-1:10'000).
Immunohistochemistry: (paraffin sections: 1:500 in TBS/Triton for 30 min. at room temperature).
Western blot: (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
|Specificity||Recognizes human adiponectin. Detects a band of ~30kDa by Western blot. Cross-reactivity with other species not tested.|
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.|
|Product Type||Monoclonal Antibody|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.|
|Use/Stability||Stable for at least 1 year after receipt when stored at -20°C.|
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans, and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
Western blot analysis of human plasma adiponectin using anti-Adiponectin (human), mAb (HADI 773) (Prod. No. AG-20A-0001) at 0.2µg/ml.
Immunohistochemical staining of adiponectin using anti-Adiponectin (human), mAb (HADI 773) (Prod. No. AG-20A-0001) in formalin-fixed and paraffin-embedded (FFPE) human kidney tissue (10µg/ml).
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