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Chemodex
4-Methylumbelliferyl phosphate disodium salt
61
CHF
CHF 61.00
In stock
CDX-M0012-M250250 mgCHF 61.00
CDX-M0012-G0011 gCHF 177.00
| Product Details | |
|---|---|
| Synonyms | 4-MUP; 4-MU-phopahte; MUP; Disodium 4-methylumbelliferyl phosphate; 7-hydroxy-4-Methylcoumarin phosphate disodium salt |
| Product Type | Chemical |
| Properties | |
| Formula | C10H7Na2O6P |
| MW | 300.11 |
| CAS | 22919-26-2 |
| Source/Host Chemicals | Synthetic |
| Purity Chemicals | ≥97% (NMR) |
| Appearance | White to off-white powder. |
| Solubility | Soluble in water (50 mg/ml). |
| Identity | Determined by 1H-NMR. |
| Declaration | Manufactured by Chemodex. |
| Other Product Data |
Click here for Original Manufacturer Product Datasheet |
| InChi Key | WUUDJQVNZPEPKN-UHFFFAOYSA-L |
| Smiles | CC(C1=CC=C(OP([O-])([O-])=O)C=C1O2)=CC2=O.[Na+].[Na+] |
| Shipping and Handling | |
| Shipping | AMBIENT |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | Protect from light and moisture. |
| Use/Stability | Stable for at least 2 years after receipt when stored at -20°C. |
| Documents | |
| MSDS | Inquire |
| Product Specification Sheet | |
| Datasheet |
 Download PDF |
Description
4-Methylumbelliferyl phosphate (4-MUP) is a fluorogenic substrate for phosphatases (mainly alkaline). It is converted to the fluorescent product 4-methylumbelliferone (4-MU), which has an emission maximum at 445-454 nm. The excitation maximum for 4-MU is pH-dependent: 330, 370, and 385 nm at pH 4.6, 7.4, and 10.4, respectively. Therefore, this phosphatase substrate is not well suited for living cell or continuous assays since 4-MU only develops maximum fluorescence at a pH value of >10. Spectral data: λex=319nm; λem=384nm (pH 9.0). λex=360nm; λem=449nm (for reaction product).
Product References
(1) D. Robinson & P. Willcox; Biochim. Biophys. Acta 191, 183 (1969) | (2) C.J. Cornish, et al.; Am. J. Clin. Pathol. 53, 68 (1970) | (3) J.P. Chambers, et al.; Clin. Chim. Acta 80, 67 (1977) | (4) F. Watanabe, et al.; Clin. Chim. Acta 91, 273 (1979) | (5) F.A. Anthony, et al.; Anal. Biochem. 155, 103 (1986) | (6) K. Soyama, et al.; Forensic Sci. Int. 18, 195 (1981) | (7) H.J. Radzun, et al.; Clin. Chim. Acta 123, 203 (1982) | (8) F.A. Anthony, et al.; Anal. Biochem. 155, 103 (1986) | (9) K.Y. Xiao, et al.; J. Tongji Med. Univ. 13, 206 (1993) | (10) K. Bednarska, et al.; Mediators Inflamm. 2006, 19307 (2006) | (11) J.J. Weiland, et al.; Anal. Biochem. 361, 140 (2007)