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anti-Hexanoyl-Lys [HEL], mAb (5F12)

JAI-MHL-021P 20 µg INQ
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Additional Information

Properties
Clone 5F12
Isotype Mouse IgG1κ
Immunogen/Antigen N epsilon hexanoyl-keyhole limpet hemocyanin (HEL-KLH).
Application ELISA
Immunohistochemistry: Recommended concentration is 2μg/mL on paraffin sections.
Western Blot
Crossreactivity All, Human
Specificity Recognizes Hexanoyl-Lys adducts. Does not cross-react with MDA, glyoxal, methylglyoxal, 1-hexanal, 2-hexenal, 1-nonannal, 2-nonenal, 4-hudroxy-2-nonenal.
Formulation Lyophilized. Contains 10mM PBS (pH7.4), 5% sucrose, 1% BSA and 0.05% Procline 950.
Reconstitution Reconstitute with 200μL distilled water.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Origin Manufactured by JaICA.
Product Type Monoclonal Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 3 years after receipt when stored at -20°C. After reconstitution, prepare aliquots and store at -20°C.
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Product Description

Oxidative damage to lipids (lipid peroxidation) has been found to play an important role in various disease and aging processes. During early stages of lipid peroxidation, lipid hydroperoxides (LOOH) are formed. These can react additionally to form later stage end products such as malondialdehyde (MDA) and hydroxynonenal (HNE). LOOH is measured to quantify early stage or acute lipid peroxidation while MDA is commonly measured to quantify late stage or chronic lipid peroxidation. More recently, it has been reported that 13-hydroperoxyoctadecanoic acid (13-HPODE), a precursor to 13-hydroxyoctadecanoic acid (13-HODE) can react with proteins to form measurable adducts by covalently binding to specific amino acid residues. The Hexanoyl-Lysine (HEL) adduct is formed upon oxidative modification of omega-6 fatty acids such as linoleic acid, the predominant polyunsaturated fatty acid (PUFA) in the human diet, and arachidonic acid. HEL may be another useful biomarker for detecting and quantifying the earlier stages of lipid peroxidation.

Product References

  1. Preparation of a monoclonal antibody to N(epsilon)-(Hexanonyl)lysine: application to the evaluation of protective effects of flavonoid supplementation against exercise-induced oxidative stress in rat skeletal muscle: Y. Kato, et al.; BBRC 274, 389 (2000) [Development and characterization of anti-HEL monoclonal antibody]
  2. Immunohistochemical detection of oxidative stress biomarkers, dityrosine and N(epsilon)-(hexanoyl)lysine, and C-reactive protein in rabbit atherosclerotic lesions: Y. Fukuchi, et al.; J. Atheroscler. Thromb. 15, 185 (2008) [Application to rabbit atherosclerotic lesions]
  3. A water-soluble fullerene vesicle alleviates angiotensin II-induced oxidative stress in human umbilical venous endothelial cells: R. Maeda, et al.; Hypertens. Res. 31, 141 (2008) [Application to cultured cells (HUVECs)]
  4. Differential Effects of High Glucose and Methylglyoxal on Viability and Polyol Metabolism in Immortalized Adult Mouse Schwann Cells: K. Sango, et al.; Open Diabetes J. 1, 1 (2008) [Application to cultured cell from mouse]
Immunohistochemical staining of cisplatin-treated rat kidney using anti-HEL, mAb (5F12).
Immunohistochemical staining of cisplatin-treated rat kidney using anti-HEL, mAb (5F12).
Immunohistochemical staining of human atherosclerotic lesions using anti-HEL, mAb (5F12).
Immunohistochemical staining of human atherosclerotic lesions using anti-HEL, mAb (5F12).
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