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anti-Dibromo-tyrosine [DiBrY], mAb (3A5)

JAI-MBY-020P 20 µg INQ
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Additional Information

Properties
Clone 3A5
Isotype Mouse IgG1κ
Immunogen/Antigen Brominated keyhole limpet hemocyanin.
Application ELISA
Immunohistochemistry: Recommended concentration is 10μg/mL
Western Blot
Crossreactivity All, Human
Specificity Recognizes dibromotyrosine (DiBrY), N-acetylated dichloro tyrosine (NAcDiClY), diiodo tyrosine (DiIY), N-bromosuccinimide-treated BSA, HOCl-treated BSA and dibromo-tyrosone conjugated BSA (halogene-modified tyrosine at both 3- and 5-position). Does not cross-react with dityrosine-conjugated BSA, peroxynitrite-treated BSA, tyrosine (Y), chrolotyrosine(ClY), 3-3’-dityrosine (DiY), 3-aminotyrosine (NH2Y), 3-nitrotyrosine (NO2Y), phenylalanine (Phe), o-phosphotyrosine, 3,4-dihydroxyphenylacetic acid (DOPA) and 2-hydroxy-4-phenylacetic acid. Weakly cross-reacts with Bromotyrosine (BrY).
Purity Detail Protein A purified.
Formulation Lyophilized. Contains 10mM Phosphate buffered saline (pH7.4).
Reconstitution Reconstitute with 200μL of distilled water.
Other Product Data Click here for Original Manufacturer Product Datasheet
Our product description may differ slightly from the original manufacturers product datasheet.
Origin Manufactured by JaICA.
Product Type Monoclonal Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice Avoid freeze/thaw cycles.
Use/Stability Stable for at least 3 years after receipt when stored at -20°C.
Documents
Protocols Download Document Download

Product Description

Neutrophils and eosinophils play an important role in the defence system against microbial infection. Myeloperoxidase (MPO) and eosinophil peroxidase (EPO) are known to catalyze formation of hypochlorous acid (HOCl) and hypobromous acid (HOBr). These reactive intermediates may react with proteins, lipids and nucleotides, and it has been reported to form tyrosine halogenation such as dibromotyrosine (DiBrY). DiBrY is a Br-modified tyrosine at 3- and 5- position, which is one of the major oxidative products derived from neutrophil myeloperoxidase. DiBrY may be a novel biomarker for tissue damage which is related to inflammatory and allergic disorders.

Product References

  1. Immunogenicity of a brominated protein and successive establishment of a monoclonal antibody to dihalogenated tyrosine: Y. Kato, et al.; Free Rad. Biol. Med. 38, 24 (2005)
Immunohistochemical staining using anti-Dibromo-tyrosine, mAb (3A5).
Immunohistochemical staining using anti-Dibromo-tyrosine, mAb (3A5).
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