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8-OHdG Check ELISA Kit (High Sensitivity)

JAI-KOG-HS10E 1 Kit INQ
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Additional Information

Product Data
Synonyms 8-hydroxy-2' -deoxyguanosine
Properties
Application Quantitative ELISA
Specificity Specific for 8-OHdG. Has been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Crossreactivity All
Quantity 96 wells
Range 0.125 to 10 ng/mL
Sample Type Plasma, Serum, Urine
Assay Type Competitive
Detection Type Colorimetric
Other Product Data This ELISA Kits works also for DNA extracted from cultured cells or tissues.
Detection Guide for Serum/Plasma
Detection Guide for tissue samples or cultured cells
Origin Manufactured by JaICA.
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download Document Download
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Product Description

8-hydroxy-2'-deoxyguanosine (8-OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals (incl. singlet oxygen and direct photodynamic action) that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8-OHdG is popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA and can be detected in tissue, serum, urine and other biomaterials.

Product References

  1. Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA: S. Saito, et al.; Res. Commun. Mol. Pathol. Pharmacol. 107, 39 (2000) [Development and assessment of 8-OHdG Check ELISA]
  2. Discrepancies in the measurement of UVC-induced 8-oxo-2'-deoxyguanosine: Implications for the analysis of oxidative DNA damage: M.D. Evans, et al.; BBRC 259, 374 (1999) [High correlation between HPLC-ECD when 8-OHdG in DNA samples]
  3. Mutagenicity of reactive oxygen and nitrogen species as detected by co-culture of activated inflammatory leukocytes and AS52 cells: H.W. Kim, et al.; Carcinogenesis 24, 235 (2003) [Detection of 8-OHdG in cultured cells]
  4. Significance of 8-hydroxy-2'-deoxyguanosine levels in patients with idiopathic dilated cardiomyopathy: E. Watanabe, et al.; J. Card. Fail. 12, 527 (2006) [Serum 8-OHdG in IDC patients]
  5. Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage: T. Shiihara, et al.; J. Neurol. Sci. 250, 159 (2006) [Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF)]
  6. Effects of caloric restriction on post-spawning death of ayu: R. Nagasaka, et al.; Exp. Gerontol. 40, 556 (2005) [Detection of 8-OHdG in fish tissues (brain and liver)]
  7. Salivary 8-OHdG: a useful biomarker for predicting severe ED and hypogonadism: M. Yasuda, et al.; J. Sex. Med. 5, 1482 (2008) [Detection of 8-OHdG in saliva]
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