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8-OHdG Check ELISA Kit

JAI-KOG-200SE 1 Kit INQ
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Additional Information

Product Data
Synonyms 8-hydroxy-2' -deoxyguanosine
Properties
Application Quantitative ELISA
Specificity Specific for 8-OHdG. Has been tested to 8-OHdG analogues (guanosine(G),7-methyl-G, 6-SH-G, 8-Bromo-G, dA, dC, dT, dI, dU, dG, O6-methyl-dG,8-OHdA, guanine(Gua),O6-methyl-Gua, 8-OH-Gua, uric acid, urea, creatine, creatinine, 8-sulfhydryl-G, 8-OH-G).
Crossreactivity All
Quantity 96 wells
Range 0.5 to 200 ng/mL
Sample Type Urine
Assay Type Competitive
Detection Type Colorimetric
Other Product Data This ELISA Kit is suitable for urine and serum samples from animal and human. However, for human serum, tissue and cultured cells, we recommend 8-OHdG Check ELISA Kit (High Sensitivity) (JAI-KOG-HS10E).

Detection Guide for Serum/Plasma
Detection Guide for Urine
Origin Manufactured by JaICA.
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download Document Download
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Product Description

8-hydroxy-2'-deoxyguanosine (8-OHdG) is a modified base that occurs in DNA due to attack by hydroxyl radicals (incl. singlet oxygen and direct photodynamic action) that are formed as byproducts and intermediates of aerobic metabolism and during oxidative stress. There is increasing evidence to support the involvement of free radical reactions in the damage of biomolecules that eventually lead to several diseases in humans, such as atherosclerosis, cerebral and heart ischemia-reperfusion injury, cancer, rheumatoid arthritis, inflammation, diabetes, aging, and neurodegenerative conditions, such as Alzheimer’s disease. 8-OHdG is popular as a sensitive, stable and integral marker of oxidative damage in cellular DNA and can be detected in tissue, serum, urine and other biomaterials.

Product References

  1. Quantitative determination of urinary 8-hydroxydeoxyguanosine (8-OH-dg) by using ELISA: S. Saito, et al.; Res. Commun. Mol. Pathol. Pharmacol. 107, 39 (2000) [Development and assessment of 8-OHdG Check ELISA]
  2. Urinary 8-hydroxy-2'-deoxyguanosine levels increase after reperfusion in acute myocardial infarction and may predict subsequent cardiac events: Y. Nagayoshi, et al.; Am. J. Cardiol. 95, 514 (2005) [Measurement of 8-OHdG in human urine]
  3. The effects of an antioxidant-supplemented beverage on exercise-induced oxidative stress: results from a placebo-controlled double-blind study in cyclists: J. Morillas-Ruiz, et al.; Eur. J. Appl. Physiol. 95, 543 (2005) [Study about exercise, antioxidant supplements and urinary 8-OHdG]
  4. Effect of a novel free radical scavenger, edaravone, on puromycin aminonucleoside induced nephrosis in rats: T. Someya, et al.; Pediatr. Nephrol. 20, 1430 (2005) [Effect of antioxidants to 8-OHdG in rat urine]
  5. Acute encephalopathy with refractory status epilepticus: Bilateral mesial temporal and claustral lesions, associated with a peripheral marker of oxidative DNA damage: T. Shiihara, et al.; J. Neurol. Sci. 250, 159 (2006) [Measurement of 8-OHdG in human urine, serum and cerebro spinal fluid(CSF)]
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