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Zinc Colorimetric Assay Kit (5-Br-PAPS Method)

JAI-CZN-001 100 tests INQ
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Additional Information

Properties
Application Quantitative ELISA
Specificity Specific to Zn2+.
Crossreactivity All
Quantity Enough reagents for 100 tests.
Range 4 - 1000μg/dL
Sample Type Cell Lysate, Plasma, Saliva, Serum, Tissue Supernatant, Urine
Detection Type Colorimetric
Kit Contains 1 x 23 ml    R-1   Buffer (ready to use)
1 x 0.5 ml   R-2   Chelate color (ready to use)
1 x 1.2 ml   STD  Zinc (Zn) standard 200 µg/dL (ready to use)
Other Product Data This MC-Reagent Zinc Assay Kit is a direct colorimetric assay based on the 5-Br-PAPS method without deproteinization of the sample. The zinc determination is based on the reaction of zinc with 5-Br-PAPS at alkaline pH in a buffered media, which forms a stable colored complex. The color intensity is proportional to the zinc concentration in the sample. Absorbance of the Zn2+-complex is measured at 560nm. Wavelength range of sensitivity: 550 ~ 580nm.

Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples
Origin Manufactured by JaICA.
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
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Product Description

Zinc (Zn2+) is an essential trace element, vital for the health of animals, plants, and microorganisms . Zinc is found in hundreds of enzymes, is stored and transferred in metallothioneins, and serves as structural ions in transcription factors. Zinc is often coordinated to the sides chains of amino acids, such as aspartic acid, glutamic acid, histidine and cysteine and is involved in nucleid acid and protein synthesis. It is therefore a necessary complement for cell replication. In humans, zinc interacts with a variety of organic ligands, and has roles in nucleic acid metabolism, apoptosis, neurological development, signal transduction, and gene expression. Zinc deficiency is related to skin lesions, irritability, loss of hair, growth retardation and impaired immunological functions.

Product References

  • The therapeutic effect on bone mineral formation from biomimetic zinc containing tricalcium phosphate (ZnTCP) in zinc-deficient osteoporotic mice: J. Chou, et al.; PLoS One 8, e71821 (2013)
  • A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase: Y. Sakurai, et al.; J. Biol. Chem. 289, 20139 (2014)
This assay shows, A) good linearity for full assay range and B) good correlation with ICP (known concentration).
This assay shows, A) good linearity for full assay range and B) good correlation with ICP (known concentration).
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