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Iron Colorimetric Assay Kit (Nitroso-PSAP Method)

JAI-CFE-010 100 tests INQ
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Additional Information

Application Quantitative ELISA
Specificity Specific to Fe2+ and Fe3+.
Crossreactivity All
Quantity Enough reagents for 100 tests.
Range 10 - 1'000μg/dL
Sample Type Cell Lysate, Plasma, Saliva, Serum, Tissue Supernatant, Urine
Detection Type Colorimetric
Kit Contains 1 x 16 ml    R-1   Buffer (ready to use)
1 x 7 ml      R-2   Buffer (ready to use)
1 x 0.7 ml   R-3   Chelate color (ready to use)
1 x 1.5 ml   STD  Iron (Fe) standard 200 µg/dL (ready to use)
Other Product Data This MC-Reagent Iron Assay Kit is a direct colorimetric assay based on the Nitroso-PSAP method without deproteinization of the sample. A weak acid buffer dissociates iron from the transferrin-iron complex and is reduced by a reductant (Ferric >> Ferrous). Ferrous ions form a complex with the chromogen Nitroso-PSAP. The color intensity is proportional to the iron concentration in the sample. Absorbance of the Fe2+-complex is measured at 750nm. Wavelength range of sensitivity: 740 ~ 760nm.

Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples
Origin Manufactured by JaICA.
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Manual Download Document Download

Product Description

Iron is a mineral (functioning as an enzyme cofactor) that plays an essential role in many biological processes. It is essential to nearly all known organisms. As a transition element it can form a range of oxidation states, the most common being Fe2+ (or ferrous iron) and Fe3+ (or ferric iron). Ingested iron is mainly absorbed in the form of Fe2+. The trivalent form and the heme-bound Fe2+-component of iron is reduced by vitamin C. Before passing into the plasma, it is oxidized by ceruloplasmin to Fe3+ and bound to transferrin to form a transferrin-iron complex. Iron is generally stored in the centre of metalloproteins, in the heme complex, and in oxygen carrier proteins. Iron-containing proteins participate in many reactions, often utilizing transitory changes in the oxidation state of iron to carry out chemical reactions. Iron is important for redox reactions, oxygen transport (e.g. hemoglobin), short-term oxygen storage (e.g. myoglobin) and energy generation. Iron deficiency has many adverse consequences, including anemia, hemochromatosis, chronic renal disease and in children, behavioral and learning disorders. Iron excess is toxic to the body, harming the heart, liver, skin, pancreatic islet beta cells, bones, joints, and pituitary gland. Maintaining proper iron balance is essential for maintaining homeostasis and health.

Product References

  • An automated method for measurement of serum iron and unsatur- ated iron binding capacity using nitroso-PSAP: M. Yamada, et al.; Jpn. J. Lab. Auto. 13, p659 (1988)
  • FecA1, a bacterial iron transporter, determines the survival of Helicobacter pylori in the stomach: H. Tsugawa, et al.; Free Radic. Biol. Med. 52, 1003 (2012)
  • Estrogen Regulates Hepcidin Expression via GPR30-BMP6-Dependent Signaling in Hepatocytes: Y. Ikeda, et al.; PLoS One 7, e40465 (2012)
  • GRK6 deficiency in mice causes autoimmune disease due to impaired apoptotic cell clearance; M. Nakaya; Nat. Comm. 4, 1532 (2013)
  • Superparamagnetic nanoparticle clusters for cancer theranostics combining magnetic resonance imaging and hyperthermia treatment: K. Hayashi, et al.; Theranostics 3, 366 (2013)
This assay shows, A) good linearity for full assay range and B) good correlation with ICP (known concentration).
This assay shows, A) good linearity for full assay range and B) good correlation with ICP (known concentration).
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