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Copper Colorimetric Assay Kit (3,5-DiBr-PAESA Method)

JAI-CCU-400 100 tests INQ
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Additional Information

Properties
Application Quantitative ELISA
Specificity Specific to Cu2+ and Cu+.
Crossreactivity All
Quantity Enough reagents for 100 tests.
Range 3 - 400μg/dL
Sample Type Cell Lysate, Plasma, Saliva, Serum, Tissue Supernatant, Urine
Detection Type Colorimetric
Kit Contains 1 x 24 ml    R-1   Buffer (with reductant) (ready to use)
1 x 0.5 ml   R-2   Chelate color (ready to use)
1 x 1.2 ml   STD  Copper (Cu) standard 200 µg/dL (ready to use)
Other Product Data This MC-Reagent Copper Assay Kit is a direct colorimetric assay based on the 3,5-DiBr-PAESA method without deproteinization of the sample. A week acid buffer dissociates copper from the ceruloplasmin-copper complex and is reduced by ascorbic acid (Cu2+ >> Cu+). Cu+ ions form a blue colored complex with the chromogen 3,5-DiBr-PAESA. The color intensity is proportional to the copper concentration in the sample. Absorbance of the Cu+-complex is measured at 580nm. Wavelength range of sensitivity: 570 ~ 590nm.

Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples
Origin Manufactured by JaICA.
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download Document Download

Product Description

Copper (Cu+) is an essential trace element (i.e., micronutrient) and cofactor. 95% of copper in plasma is in complex with enzymes, mostly with ceruloplasmin, several oxidases and dismutases, which play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of haemoglobin and elastin. The micronutrient is necessary for the proper growth, development, and maintenance of bone, connective tissue, brain, heart, and many other body organs. Copper is involved in the formation of red blood cells, the absorption and utilization of iron, the metabolism of cholesterol and glucose, and the synthesis and release of life-sustaining proteins and enzymes. These enzymes in turn produce cellular energy and regulate nerve transmission, blood clotting, and oxygen transport. Copper stimulates the immune system to fight infections, to repair injured tissues, and to promote healing. Copper also helps to neutralize "free-radicals", which can cause severe damage to cells. Copper deficiency lowers the anti-oxidant function in vivo and has been associated with mental retardation, depigmentation, anemia, hypotonia and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as cardiopathy, osteoporosis, osteoarthritis, Menkes syndrome and Wilson's disease. Acute copper toxicity can cause gastroenteritis and acute renal failure.

Product References

  • Enhanced biofilm formation and/or cell viability by polyamines through stimulation of response regulators UvrY and CpxR in the two-component signal transducing systems, and ribosome recycling factor: A. Sakamoto, et al.; Int. J. Biochem. Cell Biol. 44, 1877 (2012)
  • A Primary Role for Disulfide Formation in the Productive Folding of Prokaryotic Cu,Zn-superoxide dismutase: Y. Sakurai, et al.; J. Biol. Chem. 289, 20139 (2014)
This assay shows, A) good linearity for full assay range and B) good correlation with ICP (known concentration).
This assay shows, A) good linearity for full assay range and B) good correlation with ICP (known concentration).
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