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Calcium Colorimetric Assay Kit (O-Cresolphthalein Method)

JAI-CCA-030 200 tests INQ
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Additional Information

Properties
Application Quantitative ELISA
Specificity Specific to Ca2+.
Crossreactivity All
Quantity Enough reagents for 200 tests.
Range 0.2 - 30mg/dL
Sample Type Cell Lysate, Plasma, Saliva, Serum, Tissue Supernatant, Urine
Detection Type Colorimetric
Kit Contains 1 x 38 ml    R-1   Buffer (ready to use)
1 x 10  ml   R-2   Chelate color (ready to use)
1 x 1.0 ml   STD  Calcium (Ca) standard 10mg/dL (ready to use)
Other Product Data This MC-Reagent Calcium Assay Kit is a direct colorimetric assay based on the O-Cresolphtalein (OCPC) method without deproteinization of the sample. The calcium determination is based on the reaction of calcium with O-Cresolphthalein complexone in alkaline solution, which yields a violet colored complex. The intensity of the color formed is proportional to the calcium concentration in the sample. Absorbance of the Ca2+-O-Cresolphtalein complex is measured at 570nm. Wavelength range of sensitivity: 560 ~ 590nm.

Features of this Assay: Quick & Easy to use • Species independent • Highly sensitive, stable and suitable for high-throughput testing • No toxic substances • For cell lysates, serum, plasma and wide variety of biological samples
Origin Manufactured by JaICA.
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice Do not freeze.
Use/Stability 12 months after the day of manufacturing. See expiry date on ELISA Kit box.
Documents
Manual Download Document Download

Product Description

Calcium is the most abundant mineral, essential for all living organisms, where Ca2+ sequestration and release into and out of the cytoplasm functions as a signal for many cellular processes. 99% is present as calcium hydroxyapatite in bones and less than 1 % is present in the extra-osseous ICS (intracellular space) or ECS (extracellular space), responsible for a myriad of important physiological effects. Calcium ion channels control the migration of calcium ions across cell membranes, permitting the activation and inhibition of a wide variety of enzymes. Calcium ions affect the contractility of the heart and the skeletal musculature and are essential for the function of the nervous system. In addition, calcium ions play an important role in blood clotting and bone mineralization. Abnormal calcium levels have been tied to neurological, endocrine, cardiovascular, digestive, metabolic, and pulmonary diseases, as well as cancer. In plasma, calcium is bound to a considerable extent to proteins (approx. 40%), 10% is in the form of incorganic complexes and 50% is present as free (ionized) calcium. Clacium homeostasis is regulated by the parathyroid hormone (PTH), calcitriol (CT) and calcitonin. Low levels of calcium are found in hypoparathyroidism, pseudohypoparathyroidism, vitamin D deficiency, malnutrition and intestinal malabsorbtion.

Product References

  1. Apoplastic mesophyll signals induce rapid stomatal responses to CO2 in Commelina communis: T. Fujita, et al.; New Phytol. 199, 395 (2013)
  2. Strawberry notch homologue 2 regulates osteoclast fusion by enhancing the expression of DC-STAMP: K. Maruyama, et al.; J. Exp. Med. 210, 1947 (2013)
  3. The pga gene cluster in Aggregatibacter actinomycetemcomitans is necessary for the development of natural competence in Ca(2+) -promoted biofilms: K. Hisano, et al.; Mol. Oral. Microbiol. 29, 79 (2014)
This assay shows, A) good linearity for full assay range and B) good correlation with ICP (known concentration).
This assay shows, A) good linearity for full assay range and B) good correlation with ICP (known concentration).
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