Additional Information
| Product Data | |
|---|---|
| Synonyms | FliC |
| Properties | |
| Source/Host | Salmonella typhimurium strain 14028 |
| Crossreactivity | Human, Mouse |
| Specificity | Binds to human and mouse TLR5 (Toll-like receptor 5). |
| Biological Activity | Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, β-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivo assays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice.
TLR5 can be stimulated in vitro by Flagellin at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse. |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data | UniProt link P06179: Flagellin (Salmonella typhimurium) |
| Product Type | Protein |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS | 
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Product Description
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Product References
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Flagellin (AG-40B-0095) activates TLR5.
Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
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