Adiponectin (human) ELISA Kit

  • AG-45A-0001EK-KI0196 wells INQ
  • AG-45A-0001PP-KI015 x 96 wells INQ
  • AG-45A-0001TP-KI012 x 96 wells INQ
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96 wells, INQ
2 x 96 wells, INQ
5 x 96 wells, INQ

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Additional Information

Product Data
Synonyms ACRP30; AdipoQ; apM1; GBP28; Adipocyte complement related protein of 30kDa
Properties
Application Quantitative ELISA
Specificity Detects human adiponectin. Does not cross-react with mouse adiponectin, rat adiponectin, human resistin, human RELM-β or human leptin.
Crossreactivity Human
Quantity 1 x 96 wells
2 x 96 wells (Twin Plex)
5 x 96 wells (Penta Plex)
Sensitivity 100pg/ml
Range 0.5 to 32ng/ml
Sample Type Cell Culture Supernatant, Plasma, Serum, Urine
Assay Type Sandwich
Detection Type Colorimetric
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage +4°C
Handling Advice After standard reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Plate and reagents should reach room temperature before use.
Documents
Manual Download Document Download
Manual TwinPlex Download Document Download
Manual PentaPlex Download Document Download
MSDS Download Document Download

Product Description

Adiponectin [ACRP30; AdipoQ] is a promising biomarker of insulin resistance and type 2 diabetes mellitus (T2DM) but also as a potential target for management of the metabolic syndrome. It is a very robust marker that is not prone to degradation or acute inflammatory challenges, is present in relatively high concentrations in the peripheral circulation, and can be collected by a variety of methods. The benefits of using adiponectin assays in clinical settings include, (a) prediction of risk of diabetes and metabolic status and (b) providing a tool to monitor metabolic improvements. Adiponectin exerts anti-atherogenic and anti-inflammatory properties and may be important as a biomarker for obesity-related cardiovascular disease (CVD). New findings showed urinary adiponectin excretion as an independent new biomarker of microvascular and macrovascular damage in T2DM and suggested it as a very promising tool for early cardiovascular disease risk assessment. Adiponectin serum level was also described as a good biomarker of colorectal adenoma, this being related to the positive correlation between obesity and increased risk of cancer at various sites (colorectal, breast, prostate and endometrium).

Product References

  1. Plasma adiponectin levels in postmenopausal women with or without long-term hormone therapy: J. Ima; Maturitas 54, 65 (2006)
  2. Association between hypoadiponectinemia and cardiovascular risk factors in nonobese healthy adults. J.A. Im, et al.; Metabolism 55, 1546 (2006)
  3. Relationship of serum adiponectin and resistin levels with breast cancer risk: J.H. Kang, et al.; J. Korean Med. Sci. 22, 117 (2007)
  4. Improved insulin sensitivity and adiponectin level after exercise training in obese Korean youth: E.S. Kim, et al.; Obesity 15, 3023 (2007)
  5. Associations of adiponectin with sex hormone-binding globulin levels in aging male and female populations: T. Yasui, et al.; Clin. Chim. Acta 386, 69 (2007)
  6. Crosstalk between high-molecular-weight adiponectin and T-cadherin during liver fibrosis development in rats: K. Asada, et al.; Intl. J. Mol. Med. 20, 725 (2007)
  7. Retinol binding protein 4, low birth weight-related insulin resistance and hormonal contraception: A. Zugaro, et al.; Endocrine 32, 166 (2007)
  8. Leptin is Associated with Endothelial Dysfunction in Healthy Obese Premenopausal Women: K. Kwon, et al.; Kor. Circ. J. 37, 251 (2007)
  9. Correlation between estrogens and serum adipocytokines in premenopausal and postmenopausal women: S.C. Hong, et al.; Menopause 14, 835 (2007)
  10. Transgenic mice expressing nuclear sterol regulatory element-binding protein 1c in adipose tissue exhibit liver histology similar to nonalcoholic steatohepatitis: H. Nakayama, et al.; Metabolism 56, 470 (2007)
  11. Insulin-sensitizing effects of exercise on Adiponectin and Retinol Binding Protein-4 concentrations in young and middle-aged women: S. Lim, et al.; J. Clin. Endocrinol. Metab. 93, 2263 (2008)
  12. Retinol Binding Protein-4 elevation is associated with serum TSH level independently of obesity in elderly subjects with normal glucose tolerance: S. H. Choi, et al.; J. Clin. Endocrinol. Metab. 93, 2313 (2008)
  13. Fat in liver/muscle correlates more strongly with insulin sensitivity in rats than abdominal fat: S. Lim, et al.; Obesity 17, 188 (2008)
  14. Obesity-related promotion of aberrant crypt foci in DMH-treated obesis Zucker rats correlates with dyslipidemia rather than hyperinsulinemia: T.C. Koch, et al.; Eur. J. Nutr. 47, 161 (2008)
  15. Changes of Serum Adiponectin Levels in Murine Experimental Sparganosis: H.J. Yang; Kor. J. Parasitol. 46, 91 (2008)
  16. Mas Deficiency in FVB/N mice produces marked changes in lipid and glycemic metabolism: S.H. Santos, et al.; Diabetes 57, 340 (2008)
  17. Weight Loss with a Low-Carbohydrate, Mediterranean, or Low-Fat Diet: I. Shai, et al.; N. Engl. J. Med. 359, 229 (2008)
  18. Adipose-specific knockout of raptor results in lean mice with enhanced mitochondrial respiration: P. Polak, et al.; Cell Metab. 8, 399 (2008)
  19. Serum Adipocyte Fatty Acid-Binding Protein Levels Are Associated With Nonalcoholic Fatty Liver Disease in Type 2 Diabetic Patients: J.H. Koh, et al.; Diab. Care 32, 147 (2009)
  20. Elevated serum g-glutamyltransferase levels are independently associated with insulin resistance in non-diabetic subjects: J.Y. Shin, et al.; Diabetes Res. Clin. Pract. 84, 152 (2009)
  21. The Lou/C rat: A model of spontaneous food restriction associated with improved insulin sensitivity and decreased lipid storage in adipose tissue: C. Veyrat-Durebex, et al.; Am. J. Physiol. Endocrinol. Metab. 296, E1120 (2009)
  22. MicroRNA Expression in Human Omental and Subcutaneous Adipose Tissue: N. Klöting, et al.; PLoS One 4, e4699 (2009)
  23. Adipokines and Systemic Lupus Erythematosus: Relationship with Metabolic Syndrome and Cardiovascular Disease Risk Factors: M. Vadacca, et al.; J. Rheumatol. 36, 295 (2009)
  24. Relationship between serum adipocytokine levels and metabolic syndrome in menopausal women: H.T. Park, et al.; Gynecol. Endocrinol. 25, 27 (2009)
  25. Circuit resistance exercise improves glycemic control and adipokines in females with type 2 diabetes mellitus: S. Kang, et al.; JSSM 8, 682 (2009)
  26. Relationship between genotype and serum levels of adipokines and bone mineral density in type 2 diabetes mellitus patients: K. Mirzaei, et al.; Iran. J. Diab. Lip. Dis. 8, 77 (2009)
  27. Combined Impact of Adiponectin and Retinol-binding Protein 4 on Metabolic Syndrome in Elderly People: The Korean Longitudinal Study on Health and Aging: S. Lim, et al.; Obesity 18, 826 (2010)
  28. Body weight control by a high-carbohydrate/low-fat diet slows the progression of diabetic kidney damage in an obese, hypertensive, type 2 diabetic rat model: S. Ohtomo, et al.; J. Obes. 2010, 136502 (2010)
  29. Combined effects of body mass index and cardio/respiratory fitness on serum vaspin concentrations in Korean young men: J.K. Chou, et al.; Eur. J. Appl. Physiol. 108, 347 (2010)
  30. Adiponectin, Resistin and Leptin Response to Dietary Intervention in Diabetic Nephropathy: L. Kozlowska, et al.; J. Ren. Nutr. 20, 255 (2010)
  31. Possible involvement and the mechanisms of excess trans-fatty acid consumption in severe NAFLD in mice: N. Obara, et al.; J. Hepatol. 53, 326 (2010)
  32. Improved lipid and glucose metabolism in transgenic rats with increased circulating angiotensin-(1-7): S.H. Santos, et al.; Arterioscler. Thromb. Vasc. Biol. 30, 953 (2010)
  33. Dietary sphingolipids ameliorate disorders of lipid metabolism in Zucker fatty rats: K. Yunoki, et al.; J. Agric. Food Chem. 58, 7030 (2010)
  34. Association of insulin resistance with anti-Mullerian hormone levels in women without polycystic ovary syndrome (PCOS): H.T. Park, et al.; Clin. Endocrinol. (Oxf) 72, 26 (2010)
  35. Effects of body composition, leptin, and adiponectin on bone mineral density in prepubertal girls: Y.J. Rhie, et al.; J. Korean Med. Sci. 25, 1187 (2010)
  36. Adiponectin is produced by lymphocytes and is a negative regulator of granulopoiesis: L.J. Crawford, et al.; J. Leukoc. Biol. 88, 807 (2010)
  37. A nonthiazolidinedione peroxisome proliferator-activated receptor / dual agonist CG301360 alleviates insulin resistance and lipid dysregulation in db/db mice: H.W. Jeong, et al.; Mol. Pharmacol. 78, 877 (2010)
  38. Adipokine pattern in subjects with impaired fasting glucose and impaired glucose tolerance in comparison to normal glucose tolerance and diabetes: A. Tönjes, et al.; PLoS One 5, e13911 (2010)
  39. Body weight control by a high-carbohydrate/low-fat diet slows the progression of diabetic kidney damage in an obese, hypertensive, type 2 diabetic rat model: S. Ohtomo, et al.; J. Obes. 2010, 136502 (2010)
  40. Serum levels of CK18 M30 and leptin are useful predictors of steatohepatitis and fibrosis in paediatric NAFLD: E. Fitzpatrick, et al.; J. Pediatr. Gastroenterol. Nutr. 51, 500 (2010)
  41. Resistin, adiponectin and visfatin; can adipocytokines predict gestational diabetes mellitus and early post partum metabolic syndrome?: A. Hossein-nezhad, et al.; Iran. J. Diab. Lip. Dis. 9, 6 (2010)
  42. Combined effects of body mass index and cardio/respiratory fitness on serum vaspin concentrations in Korean young men: J.K. Cho, et al.; Eur. J. Appl. Physiol. 108, 347 (2010)
  43. A nonthiazolidinedione peroxisome proliferator-activated receptor α/γ dual agonist CG301360 alleviates insulin resistance and lipid dysregulation in db/db mice: H.W. Jeong, et al.; Mol. Pharmacol. 78, 877 (2010)
  44. Organotypic culture of human bone marrow adipose tissue: K. Uchihashi, et al.; Pathol. Int. 60, 259 (2010)
  45. Phenotypic and genetic variation in leptin as determinants of weight regain: G. Erez, et al.; Int. J. Obes. (Lond) (Epub ahead of print) (2010)
  46. ADIPOQ SNP45 associated with lean body mass in physically active normal weight adolescent girls: C.L. Passariello, et al.; Am. J. Hum. Biol. 22, 813 (2010)
  47. Adiponectin, resistin and leptin response to dietary intervention in diabetic nephropathy: L. Kozłowska, et al.; J. Ren. Nutr. 20, 255 (2010)
  48. Body weight control by a high-carbohydrate/low-fat diet slows the progression of diabetic kidney damage in an obese, hypertensive, type 2 diabetic rat model: S. Ohtomo, et al.; J. Obes. 2010, 136502 (2010)
  49. Adipocytokines and bone mineral density in adolescent female athletes: R. Gruodyte, et al.; Acta Paediatr. 99, 1879 (2010)
  50. Effect of green tea extract on bone turnover markers in type 2 diabetic patients; A double- blind, placebo-controlled clinical trial study: K. Mirzaei, et al.; DARU J. Pharm. Sci. 17 (Suppl 1), 38 (2010)
  51. Apolipoprotein B/A1 ratio is associated with free androgen index and visceral adiposity and may be an indicator of metabolic syndrome in male children and adolescents: Y.H. Lee, et al.; Clin. Endocrinol. (Oxf) 74, 579 (2011)
  52. Circulating Nampt and RBP4 levels in patients with carotid stenosis undergoing carotid endarterectomy (CEA): G. Aust, et al.; Clin. Chim. Acta 412, 1195 (2011)
  53. Serum visfatin is associated with type 2 diabetes mellitus independent of insulin resistance and obesity: A. Esteghamati, et al.; Diabetes Res. Clin. Pract. 91, 154 (2011)
  54. Role of AMP-activated Protein Kinase and Adiponectin during Development of Hepatic Steatosis in High-fat Diet-induced Obesity in Rats: S.K. Ha, et al.; J. Comp. Pathol. (Epub ahead of print) (2011)
  55. Adipokines, insulin resistance, metabolic syndrome, and breast cancer recurrence: a cohort study: S.W. Oh, et al.; Breast Cancer Res. 13, R34 (2011)
  56. Plasma visfatin and adiponectin concentrations in physically active adolescent girls: relationships with insulin sensitivity and body composition variables: J. Jürimäe, et al.; JPEM 24, 419 (2011)
  57. Relationship between inflammation biomarkers, antioxidant vitamins, and bone mineral density in patients with metabolic syndrome: Y. Lee, et al.; Nutr. Res. Pract. 5, 150 (2011)
  58. Clinically silent adrenal adenomas - their relation to the metabolic syndrome and to GNB3 C825T gene polymorphism: I. Lazurova, et al.; Wien. Klin. Wochenschr. 123, 618 (2011)
  59. Optimal cut points of waist circumference (WC) and visceral fat area (VFA) predicting for metabolic syndrome (MetS) in elderly population in the Korean Longitudinal Study on Health and Aging (KLoSHA): S. Lim, et al.; Arch. Gerontol. Geriatr. (Epub ahead of print) (2011)
  60. Effects of aerobic exercise training on C1q tumor necrosis factor α-related protein isoform 5 (myonectin): association with insulin resistance and mitochondrial DNA density in women: S. Lim, et al.; J. Clin. Endocrinol. Metab. 97, E88 (2012)
  61. Genetic effects of adiponectin single nucleotide polymorphisms on the clustering of metabolic risk factors in young Korean adults: J.Y. Lee, et al.; Eur. J. Appl. Physiol. 112, 623 (2012)
  62. The effects of detraining on blood adipokines and antioxidant enzyme in Korean overweight children: J. Woo, et al.; Eur. J. Pediatr. 171, 235 (2012)
  63. Plasma adiponectin elevation in elderly individuals with subsyndromal depression: H.G. Jeong, et al.; Psychoneuroendocrinol. (Epub ahead of print) (2011) 
  64. Leptin, adiponectin and vascular stiffness parameters in women with systemic lupus erythematosus: M. Vadacca, et al.; Intern. Emerg. Med. (Epub ahead of print) (2011)
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