EnhancedTRAIL, Soluble (human) (rec.) Pack

  • AG-44B-0002-KI011 Set INQ
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Product Data
Synonyms Apo-2L; TNFSF 10; CD253
Properties
Specificity Binds to human and mouse TRAIL receptors and human osteoprotegrin (OPG).
Crossreactivity Human, Mouse
Kit Contains 1 Set
2x10μg TRAIL, Soluble (human) (rec.) (Prod. No. AG-40B-0003)
3x50μg TNF Ligands Enhancer (Prod. No. AG-35B-0001)
Other Product Data Source/Host: Produced in E. coli. A FLAG®-tag is fused at the N-terminus of the extracellular domain of human TRAIL (aa 95-281).
Concentration: 
TRAIL: 0.1mg/ml after reconstitution
TNF Ligands Enhancer: 1mg/ml after reconstitution
Purity: 
≥95% (SDS-PAGE)
Formulation:
Lyophilized. Contains PBS.
Endotoxin Content:
TRAIL: <0.1EU/µg purified protein (LAL test; Lonza).
TNF Ligands Enhancer: <0.1EU/µg purified protein (LAL test; Lonza).
Reconstitution:
TRAIL: reconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
TNF Ligands Enhancer: reconstitute each vial with 50µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
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Product Description

Induces apoptosis in a concentration range of 5-50ng/ml in the presence of 1μg/ml of TNF Ligands Enhancer (Prod. No. AG-35B-0001).

Product References

  1. Sensitivity to TRAIL/APO-2L-mediated apoptosis in human renal cell carcinomas and its enhancement by topotecan: M. Dejosez, et al.; Cell Death Differ. 7, 1127 (2000)
  2. Three adenovirus E3 proteins cooperate to evade apoptosis by tumor necrosis factor-related apoptosis-inducing ligand receptor-1 and -2: C.A. Benedict et al.; J. Biol. Chem. 276, 3270 (2001)
  3. The anti-apoptotic protein BAG-3 is overexpressed in pancreatic cancer and induced by heat stress in pancreatic cancer cell lines: Q. Liao, et al.; FEBS Lett. 503, 151 (2001)
  4. Caspase-10 is an initiator caspase in death receptor signaling: J. Wang, et al.; PNAS 98, 13884 (2001)
  5. Tumor necrosis factor-related apoptosis-inducing ligand-induced death-inducing signaling complex and its modulation by c-FLIP and PED/PEA-15 in glioma cells: C. Xiao, et al.; J. Biol. Chem. 277, 25020 (2002)
  6. TRAIL and its receptors in the colonic epithelium: a putative role in the defense of viral infections: J. Strater, et al.; Gastroenterology 122, 659 (2002)
  7. TRAIL/Apo-2 ligand induces primary plasma cell apoptosis: J. Ursini-Siegel, et al.; J. Immunol. 169, 5505 (2002)
  8. Induction of TRAIL-mediated glioma cell death by human T cells: J. Dörr, et al.; J. Neuroimmunol. 122, 117 (2002)
  9. Chemotherapy enhances TNF-related apoptosis-inducing ligand DISC assembly in HT29 human colon cancer cells: S. Lacour, et al.; Oncogene 22, 1807 (2003)
  10. Human mast cells undergo TRAIL-induced apoptosis: B. Berent-Maoz, et al.; J. Immunol. 176, 2272 (2006)
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