EnhancedFasL, Soluble (human) (rec.) Pack

  • AG-44B-0001-KI011 Set INQ
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Additional Information

Product Data
Synonyms APO-1L; CD95L; CD178; TNFSF 6; Fas Ligand
Properties
Specificity Binds to human and mouse Fas (CD95; APO-1).
Crossreactivity Human, Mouse
Kit Contains 1 Set
1x10µg of FasL, Soluble (human) (rec.) (Prod. No. AG-40B-0001)
4x50µg of TNF Ligands Enhancer (Prod. No. AG-35B-0001)
Other Product Data Source/Host:
Produced in HEK 293 cells. A FLAG®-tag is fused at the N-terminus of the extracellular domain of human FasL (aa 103-281).
Concentration:
FasL: 0.1mg/ml after reconstitution
TNF Ligands Enhancer: 1mg/ml after reconstitution
Purity:
≥95% (SDS-PAGE)
Formulation:
Lyophilized. Contains PBS.
Endotoxin Content:
FasL: <0.1EU/µg purified protein (LAL test; Lonza).
TNF Ligands Enhancer: <0.1EU/µg purified protein (LAL test; Lonza).
Reconstitution:
FasL: reconstitute with 100µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
TNF Ligands Enhancer: reconstitute each vial with 50µl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions.
Product Type Kit
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability Stable for at least 6 months after receipt when stored at -20°C.
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Product Description

FasL, Soluble (human) (rec.) induces apoptosis in a concentration range of <1ng/ml in the presence of 0.1 to 1µg/ml of TNF Ligands Enhancer (Prod. No. AG-35B-0001). (Optimal conditions must be determined individually for each cell line tested)

Product References

  1. Activation of Fas by FasL induces apoptosis by a mechanism that cannot be blocked by Bcl-2 or Bcl-x(L): D.C. Huang, et al.; PNAS 96, 14871 (1999)
  2. Fas ligand-induced c-Jun kinase activation in lymphoid cells requires extensive receptor aggregation but is independent of DAXX, and Fas-mediated cell death does not involve DAXX, RIP, or RAIDD: A. Villunger, et al.; J. Immunol. 165, 1337 (2000) 
  3. Fas engagement induces the maturation of dendritic cells (DCs), the release of interleukin (IL)-1beta, and the production of interferon gamma in the absence of IL-12 during DC-T cell cognate interaction: a new role for Fas ligand in inflammatory responses: M. Rescigno, et al.; J. Exp. Med. 192, 1661 (2000) 
  4. NF-κB signals induce the expression of c-FLIP: O. Micheau, et al.; Mol. Cell. Biol. 21, 5299 (2001) 
  5. Potentiation of Fas-mediated apoptosis by an engineered glycosylphosphatidylinositol-linked Fas: P. Legembre, et al.; Cell Death Differ. 9, 329 (2002) 
  6. Multiple pathways of TWEAK-induced cell death: M. Nakayama, et al.; J. Immunol. 168, 734 (2002) 
  7. Innate direct anticancer effector function of human immature dendritic cells. II. Role of TNF, lymphotoxin-alpha(1)beta(2), Fas ligand, and TNF-related apoptosis-inducing ligand: G. Lu, et al.; J. Immunol. 168, 1831 (2002) 
  8. Induction of apoptosis in malignant pleural mesothelioma cells by activation of the Fas (Apo-1/CD95) death-signal pathway: J.H. 4th Stewart et al.; J. Thorac. Cardiovasc. Surg. 123, 295 (2002) 
  9. Hepatic natural killer cells exclusively kill splenic/blood natural killer-resistant tumor cells by the perforin/granzyme pathway: D. Vermijlen, et al.; J. Leukoc. Biol. 72, 668 (2002)
  10. Caspase-10 is recruited to and activated at the native TRAIL and CD95 death-inducing signalling complexes in a FADD-dependent manner but can not functionally substitute caspase-8: M.R. Sprick, et al.; EMBO J. 21, 4520 (2002) 
  11. An essential role for membrane rafts in the initiation of Fas/CD95-triggered cell death in mouse thymocytes: A.O. Hueber, et al.; EMBO Rep. 3, 190 (2002) 
  12. Cutting edge: SDS-stable Fas microaggregates: an early event of Fas activation occurring with agonistic anti-Fas antibody but not with Fas ligand: P. Legembre, et al.; J. Immunol. 171, 5659 (2003) 
  13. The role of p53 and Fas in a model of acute murine graft-versus-host disease: S. Yada, et al.; J. Immunol. 174, 1291 (2005) 
  14. CD1d-unrestricted human NKT cells release chemokines upon Fas engagement: M. Giroux & F. Denis; Blood 105, 703 (2005) 
  15. Amplification of CD95 activation by caspase 8-induced endosomal acidification in rat hepatocytes: R. Reinehr, et al.; J. Biol. Chem. 283, 2211 (2008) 
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