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IL-2 Superkine (Fc)

AG-40B-0111-C010 10 µg INQ
AG-40B-0111-3010 3 x 10 µg INQ
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Additional Information

Product Data
Synonyms IL-2 Superkine (human):Fc (human) (rec.); Interleukin-2; T Cell Growth Factor; TCGF; Aldesleukin; Super-2
Properties
Source/Host HEK 293 cells
Sequence The extracellular domain of human IL-2 (aa 21-153) (mutant H9 containing the mutations L80F / R81D / L85V / I 86V / I92F) is fused at the C-terminus to the Fc portion of human IgG2.
Crossreactivity Human
Application Triggers far greater antitumor responses than native IL-2 in vivo but with lower toxicity.
Specificity Binds to human and mouse IL-2R.
MW 42kDa
Purity ≥95% (SDS-PAGE)
Endotoxin Content <0.01EU/μg protein (LAL test; Lonza).
Concentration 0.1mg/ml after reconstitution.
Reconstitution Reconstitute with 100μl sterile water.
Formulation Lyophilized. Contains PBS.
Other Product Data Uniprot link P60568 : IL-2 (human)
AfCS Signaling Gateway link A006222: IL-2 (mouse)
Product Type Protein
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
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Product Description

Interleukin-2 (IL-2) is a 133 amino acid glycoprotein with one intramolecular disulfide bond and variable glycosylation. It is secreted by activated T cells and induces proliferation and maturation of activated T cells, natural killer cells and lymphokine activated killer cells. IL-2 also stimulates proliferation of antibody-producing B cells, activates neutrophils and induces mononuclear cells to secrete IFN-γ and TNF-α and -β. Moreover, studies have shown that IL-2 is required for activation-induced apoptosis, an important homeostatic mechanism in the immune system, which is involved in the maintenance of peripheral tolerance to self-antigens.

IL-2 promotes T cell proliferation and particularly naive T cells. IL-2 signaling on activated T cells is effected through a quaternary high-affinity receptor complex consisting of IL-2, IL-2Rα (CD25), IL-2Rβ and IL-2Rγ. Naive T cells are relatively insensitive to IL-2 as they only express small amounts of IL-2Rβ and IL-2Rγ. They only acquire sensitivity after CD25 expression, which captures the cytokine and presents it to the IL-2Rβ and IL-2Rγ receptors. IL-2 Superkine (Fc) is an artificial variant of IL-2 containing mutations at positions L80F / R81D / L85V / I 86V / I92F. These mutations are located in the molecule's core that acts to stabilize the structure and to give it a receptor-binding conformation mimicking native IL-2 bound to CD25. These mutations effectively eliminate the functional requirement of IL-2 for CD25 expression and elicit proliferation of T cells. Compared to IL-2, the IL-2 superkine induces superior expansion of cytotoxic T cells, leading to improved antitumour responses in vivo, and elicits proportionally less toxicity by lowering the expansion of Tregulatory cells and reducing pulmonary oedema.

Product References

  1. Exploiting a natural conformational switch to engineer an interleukin-2 'superkine': AM. Levin, et al.; Nature 484, 529 (2012)
Binding of IL-2 Superkine (Fc) (AG-40B-0111) to IL-2R&beta; (human) is increased >10 fold compared to IL-2 (human):Fc (human) .<br /> <b>Methods:</b> IL-2R&beta; (human) was coated on an ELISA plate at 1&mu;g/ml. After blocking and washing steps, indicated concentrations of IL-2 Superkine (Fc) or IL-2 (human):Fc (human) were added. Following  incubation for 1 h at RT, the binding was detected using an anti-human Fc antibody (HRP).
Binding of IL-2 Superkine (Fc) (AG-40B-0111) to IL-2Rβ (human) is increased >10 fold compared to IL-2 (human):Fc (human) .
Methods: IL-2Rβ (human) was coated on an ELISA plate at 1μg/ml. After blocking and washing steps, indicated concentrations of IL-2 Superkine (Fc) or IL-2 (human):Fc (human) were added. Following incubation for 1 h at RT, the binding was detected using an anti-human Fc antibody (HRP).
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