Additional Information
| Product Data | |
|---|---|
| Synonyms | Interleukin-36 Receptor Antagonist Protein; IL-1F5; Interleukin-1 Family Member 5; IL-1δ; Interleukin-1δ; Interleukin-1 Homolog 3; Interleukin-1-like Protein 1 |
| Properties | |
| Source/Host | E. coli |
| Sequence | Mouse IL36Ra (aa Val3-Asp156) is untagged. |
| Crossreactivity | Mouse |
| Application | Inhibition of biological activity of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9). |
| Biological Activity | Inhibits the IL-6 induced expression of IL-36α (IL-1F6), IL-36β (IL-1F8), and IL-36γ (IL-1F9) in BMDCs. 1μg/ml of IL-36Ra inhibits the activity of 10 ng to 100 ng/ml of the agonists. |
| MW | ~17kDa |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.05EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data | Uni-Prot link Q9QYY1: Interleukin-36 receptor antagonist protein |
| Product Type | Protein |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS | 
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Product Description
IL-36Ra/IL-1F5 is a highly and a specific antagonist of the IL-1 receptor-related protein 2-mediated response to IL-36α (IL-1F6), IL-36β (IL-1F8) and IL-36γ (IL-1F9). These cytokines bind to IL-36R (IL-1Rrp2) and IL-1RAcP, activating similar intracellular signals as IL-1. IL-36Ra inhibits the production of proinflammatory cytokines, including IL-12, IL-1β, IL-6, TNF-α and IL-23 induced by IL-36 in BMDC and CD4 T cells. Skin and dendritic cells are target of the IL-36 interleukins leading to a Th1 response. Recently mutations that affect the levels and the activity of IL-36Ra have been found in patients with pustular psoriasis, leading to enhanced production of inflammatory cytokines (IL-8 in particular) by keratinocytes.
IL-36Ra (aa 3-156) (mouse) (rec.) (Prod. No. AG-40B-0096) inhibits IL-36α, IL-36β, IL-36γ-dependent expression of IL-6 in mouse BMDCs.
Method: Bone marrow cells were differentiated into bone marrow-derived dendritic cells (BMDCs) using GM-CSF (20ng/ml) for 10 days. BMDCs were cultured with or without IL-36Ra, IL-36α, IL-36β, IL-36γ, or LPS at indicated concentrations for 72 h. Cell supernatants were then harvested and IL-6 levels determined by ELISA.
Method: Bone marrow cells were differentiated into bone marrow-derived dendritic cells (BMDCs) using GM-CSF (20ng/ml) for 10 days. BMDCs were cultured with or without IL-36Ra, IL-36α, IL-36β, IL-36γ, or LPS at indicated concentrations for 72 h. Cell supernatants were then harvested and IL-6 levels determined by ELISA.
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