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AdipoGen Life Sciences
Flagellin
290
CHF
CHF 290.00
In stock
AG-40B-0095-C100100 µgCHF 290.00
Figure 1: Flagellin (AG-40B-0095) activates TLR5.
Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
Method: Caco-2 cells transfected with plasmid encoding CCL20-LUC plasmid were activated for 24h with the indicated concentrations of Flagellin (Prod. No. AG-40B-0095). Luciferase assays were performed on cell lysates and luminescence was normalized to mock-treated cells.
| Product Details | |
|---|---|
| Synonyms | FliC |
| Product Type | Protein |
| Properties | |
| Source/Host | Salmonella typhimurium strain 14028 |
| Crossreactivity |
Human Mouse |
| Specificity |
Binds to human and mouse TLR5 (Toll-like receptor 5). |
| Biological Activity |
Activation of TLR5 in human epithelial cell assays based on NF-κB luciferase fusions, on expression of IL-8 (CXCL8), CCL20, inducible nitric oxide synthase, β-defensin 2 and matrilysin (MMP-7). Maturation of dendritic cells in in vitro and in vivoassays. Adjuvant activity on foreign antigens delivered by subcutaneous and intranasal routes in mice. TLR5 can be stimulated in vitro by Flagellin at a concentration of 5-50ng /ml and in vivo at a starting concentration of 0.1-1µg /mouse. |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.01EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data |
UniProt link P06179: Flagellin (Salmonella typhimurium) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
Flagellin is the subunit protein which polymerizes to form the filaments of bacterial flagella. It activates the innate immune system through the receptor Toll-like Receptor 5 (TLR5) and the intracellular protein NLRC4 (NLR family CARD domain-containing protein 4).
Product References
- Deletion of flagellin's hypervariable region abrogates antibody-mediated neutralization and systemic activation of TLR5-dependent immunity: C. Nempont, et al.; J. Immunol. 181, 2036 (2008)
- The Salmonella type-3 secretion system-1 and flagellar motility influence the neutrophil respiratory burst: T.L. Westerman, et al.; PLoS One 13, e0203698 (2018)
- FSH-blocking therapeutic for osteoporosis: S. Gera, et al.; eLife 11, e78022 (2022)
- Cornus officinalis Seed Extract Inhibits AIM2-Inflammasome Activation and Attenuates Imiquimod-Induced Psoriasis-like Skin Inflammation: S-B. Lee, et al.; Int. J. Mol. Sci. 24, 5653 (2023)
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