Additional Information
| Product Data | |
|---|---|
| Synonyms | Leukemia Inhibitory Factor; Differentiation-stimulating Factor; D Factor; Melanoma-derived LPL Inhibitor |
| Properties | |
| Source/Host | E. coli |
| Sequence | Human LIF (aa 23-202) is fused at the N-terminus to a GST-tag. |
| Crossreactivity | Human, Mouse |
| Specificity | Mouse and human embryonic stem cells. |
| Biological Activity | Maintains pluripotency of mouse and human embryonic stem cells. The LIF (GST-tagged) from AdipoGen has the same activity as cleaved LIF. |
| MW | ~46kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.05EU/μg purified protein (LAL test; Lonza). |
| Concentration | AG-40B-0093-C010/AG-40B-0093-3010: 0.1mg/ml after reconstitution. AG-40B-0093-C100: 1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data | UniProt link P15018: LIF (human) |
| Product Type | Protein |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
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Product Description
LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities also include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation and the stimulation of acute-phase protein synthesis in hepatocytes. LIF activates JAK & STAT signaling in human embryonic stem (ES) cells, but this pathway does not maintain pluripotency in these cells, which instead rely on FGF2-mediated ERK signaling. By contrast, mouse ES cells can be maintained by LIF-mediated JAK & STAT signaling. LIF binds to a high affinity heterodimeric receptor complex consisting of two proteins: LIF-R α that binds LIF with low affinity and the 130kDa (gp130) subunit that by itself does not bind LIF, but is required for high affinity binding of LIF.
Figure: Human Leukemia Inhibitory Factor (LIF) (rec.) (AG-40B-0093) maintains pluripotency of mouse ES cells.
Method: Mouse ES oct4 GFP cells were cultured for 3 days in the presence of the indicated concentrations of LIF and followed by flow cytometry analysis of the GFP expression (indicating the expression of Oct4, thus of pluripotency).
Method: Mouse ES oct4 GFP cells were cultured for 3 days in the presence of the indicated concentrations of LIF and followed by flow cytometry analysis of the GFP expression (indicating the expression of Oct4, thus of pluripotency).
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