Additional Information
| Product Data | |
|---|---|
| Synonyms | ACRP30headless:CD40L; ACRP30headless:CD154; ACRP30headless:TNFSF5; ADIPOQ-CD40L |
| Properties | |
| Source/Host | CHO cells |
| Sequence | Mouse CD40L (aa 115-260) is fused at the N-terminus to mouse ACRP30headless (aa 18-111) and a FLAG®-tag. |
| Crossreactivity | Human, Mouse |
| Specificity | Binds to human and mouse CD40. |
| Biological Activity | Induces B cells activation (as demonstrated by dose-dependent upregulation of CD86). |
| MW | ~35-40kDa |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 0.1mg/ml after reconstitution. |
| Reconstitution | Reconstitute with 100μl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Other Product Data | Uniprot link P27548: CD40L (mouse) FLAG is a registered trademark of Sigma-Aldrich Co. |
| Product Type | Protein |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability | Stable for at least 6 months after receipt when stored at -20°C. |
| Documents | |
| MSDS |
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Product Description
MegaCD40L is a high activity construct in which two trimeric CD40 ligands are artificially linked via the collagen domain of ACRP30. This construct very effectively simulates the natural membrane-assisted aggregation of CD40L in vivo. It provides a simple and equally potent alternative to CD40L+enhancer combinations. MegaCD40L has shown to suppress alum-induced IL-1β release and caspase-1 activation in a dose-, CD40- and time dependent manner, without affecting BMDM (Bone marrow-derived macrophages) viability. It also effectively suppressed the inflammasome function triggered by NLRP3 activators. The secretion of caspase-1 independent inflammatory mediators has been shown to be unaltered or even enhanced.
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