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AdipoGen Life Sciences
DLL1 (mouse):Fc (human) (rec.)
180
CHF
CHF 180.00
In stock
AG-40A-0148Y-C01010 µgCHF 180.00
AG-40A-0148Y-C05050 µgCHF 370.00
Figure 1: Induction of Hes1 with the treatment of recombinant mouse DLL1-Fc (Prod. No. AG-40A-0148Y).
A mouse preadipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Lane 1: mDLL1 (Fc protein), 0 min
Lane 2: mDLL1 (Fc protein), 10min
Lane 3: mDLL1 (Fc protein), 30min
Lane 4: mDLL1 (Fc protein), 1hr
Lane 5: mDLL1 (Fc protein), 2hr
Lane 6: mDLL1 (Fc protein), 4hr
Lane 7: mDLL1 (Fc protein), 8hr
Lane 8: mDLL1 (Fc protein), 24hr
A mouse preadipocyte cell line, 3T3L1, was stimulated with 5mg/ml of mouse DLL1-Fc as in indicated time points and each cell lysate was prepared and subjected to western blot by using anti-mouse Hes1 or GAPDH.
Lane 1: mDLL1 (Fc protein), 0 min
Lane 2: mDLL1 (Fc protein), 10min
Lane 3: mDLL1 (Fc protein), 30min
Lane 4: mDLL1 (Fc protein), 1hr
Lane 5: mDLL1 (Fc protein), 2hr
Lane 6: mDLL1 (Fc protein), 4hr
Lane 7: mDLL1 (Fc protein), 8hr
Lane 8: mDLL1 (Fc protein), 24hr
Figure 2: Adipogenesis inhibition. Macroscopic pictures.
Figure 3: Adipogenesis inhibition of 3T3L1 cells.
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1μM dexamethasone, 0.5mM IBMX, 10μg/m insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml mDLL1-Fc (Prod. No. AG-40A-0148Y) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin and stained with 0.5% filtered Oil Red O in propylene glycol.
3T3L1 cells were maintained in DMEM, supplemented with 10% fetal bovine serum, penicilin-streptomycin. Adipogenesis was initiated by adding 1μM dexamethasone, 0.5mM IBMX, 10μg/m insulin (day 0) until day 2. The medium was replaced every 2 days with new medium containing insulin in the presence or absence of 5μg/ml mDLL1-Fc (Prod. No. AG-40A-0148Y) and hCD137-Fc (Fc control). Staining with Oil Red O was typically performed on day 7. Cells were washed twice with PBS, fixed with 3.7% formalin and stained with 0.5% filtered Oil Red O in propylene glycol.
| Product Details | |
|---|---|
| Synonyms | Delta-like Protein 1; Delta 1 |
| Product Type | Protein |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence |
Extracellular domain of mouse DLL1 (aa 18-545) is fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Mouse |
| Specificity |
Interacts with Notch receptors. |
| Biological Activity |
Inhibits adipogenesis of 3T3L-1 cells. Induces Hes-1 in 3T3L-1 cells. |
| MW | ~100kDa (SDS-PAGE) |
| Purity | ≥90% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration |
After reconstitution: for 10µg size: 0.1mg/ml for 50µg size: 1 mg/ml |
| Reconstitution |
10µg size: Reconstitute with 100µl sterile water. 50µg size: Reconstitute with 50µl sterile water. |
| Formulation | Lyophilized. Contains PBS. |
| Protein Negative Control | |
| Other Product Data |
Uniprot link Q61483: DLL1 (mouse) |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice |
After reconstitution, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution. PBS containing at least 0.1% BSA should be used for further dilutions. |
| Use/Stability |
Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
 Download PDF |
| Product Specification Sheet | |
| Datasheet |
Download PDF |
Description
The Notch ligand delta-like protein 1 (DLL1) is essential for postnatal arteriogenesis and contributes to tumor progression. DLL1 is involved in differentiation and self-renewal of adipocyte stem cells. Blocks the differentiation of progenitor cells into the B cell lineage while promoting the emergence of a population of cells with the characteristics of a T cell/NK cell precursor.
Product References
- Flt-1 (Vascular Endothelial Growth Factor Receptor-1) Is Essential for the Vascular Endothelial Growth Factor-Notch Feedback Loop During Angiogenesis: J.C. Chappell, et al.; Arterioscler. Thromb. Vasc. Biol. 33, 1952 (2013)
- Mimicking The Stem Cell Niche In Diseased Muscle With A Notch Activator-conjugated 3d-construct: X. Mu, et al.; ORS Annual Meeting Transactions Presentation (2014)
- Notch Stimulates Both Self-Renewal and Lineage Plasticity in a Subset of Murine CD9High Committed Megakaryocytic Progenitors: M. Weiss-Gayet, et al.; PLoS One 11, e0153860 (2016)
- Reciprocal communication between astrocytes and endothelial cells is required for astrocytic glutamate transporter 1 (GLT-1) expression: Z. Martinez-Lozada & M.B. Robinson; Neurochem. Int. 139, 104787 (2020)
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