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Sirtuin 1 (human) (rec.) (His)

AG-40A-0117-C010 10 µg $170.00
AG-40A-0117-C050 50 µg $360.00
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Additional Information

Product Data
Synonyms SIRT1; NAD-dependent Deacetylase Sirtuin-1; SIR2-like Protein 1; SIR2L1
Properties
Source/Host E. coli
Sequence Human sirtuin 1 (aa 2-555) is fused at the N-terminus to a His-tag.
Crossreactivity Human
Biological Activity HDAC activity.
MW ~70kDa (SDS-PAGE)
Purity ≥95% (SDS-PAGE)
Purity Detail Purified using Ni-NTA, FPLC and LPS-removal column.
Endotoxin Content <1EU/μg purified protein (LAL test; Lonza).
Formulation Liquid. 0.2μm-filtered solution in 55mM TRIS-Cl, pH 8.2, containing 150mM NaCl.
Other Product Data UniProt link Q96EB6: SIRT1 (human)
Product Type Protein
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability Working aliquots are stable for up to 3 months when stored at -20°C.
Documents
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Product Description

Sirtuin 1 (SIRT1) is a NAD-dependent protein deacetylase, which regulates processes such as apoptosis and muscle differentiation by deacetylating key proteins. Sirtuin 1 emerged as critical regulator of longevity and aging in several model organisms. Furthermore, a variety of age-associated diseases such as type 2 diabetes, obesity, osteoporosis, and Alzheimer’s disease are associated with sirtuins. Sirtuin 1 has been suggested as potential biomarker for tumorigenesis.
HDAC activity (HDAC Fluorimetric Assay -AK-500 from ELS).<br /> This assay is performed in two stages. In the first stage, the HDAC(s) acts on the Substrate (10&mu;M, 50&mu;M) (total volume of 50&mu;l). The second stage, is initiated by the addition of 50&mu;l of developer, stops HDAC activity and produces the fluorescent signal. The samples are read in a microplate reading fluorimeter, capable of excitation at a wavelength in the range 350~380nm and detection of emission light in the range 440~460nm.
HDAC activity (HDAC Fluorimetric Assay -AK-500 from ELS).
This assay is performed in two stages. In the first stage, the HDAC(s) acts on the Substrate (10μM, 50μM) (total volume of 50μl). The second stage, is initiated by the addition of 50μl of developer, stops HDAC activity and produces the fluorescent signal. The samples are read in a microplate reading fluorimeter, capable of excitation at a wavelength in the range 350~380nm and detection of emission light in the range 440~460nm.
The effect of resvertrol on recombinant human Sirtuin1 activity. hSirt1 (AG-40A-0117): 1&mu;g (0.25&mu;g/ml), NAD+ : 200&mu;M Substrate: flour de Lys, 50&mu;M.
The effect of resvertrol on recombinant human Sirtuin1 activity. hSirt1 (AG-40A-0117): 1μg (0.25μg/ml), NAD+ : 200μM Substrate: flour de Lys, 50μM.
Inhibition of Sirtuin1 activity by nicotinamide. Substrate: flour de Lys, 50&mu;M, NAD+ : 200&mu;M.
Inhibition of Sirtuin1 activity by nicotinamide. Substrate: flour de Lys, 50μM, NAD+ : 200μM.
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