Additional Information
| Product Data | |
|---|---|
| Synonyms | SARS Receptor; Angiotensin-converting Enzyme 2; ACEH |
| Properties | |
| Source/Host | HEK 293 cells |
| Sequence | Signal peptide sequence and the extracellular domain of human ACE2 (aa 1-740) are fused at the C-terminus to the Fc portion of human IgG1. |
| Crossreactivity | Human |
| Biological Activity | Hydrolysis of a fluorescent peptide substrate, Mca-Y-V-A-D-A-P-K(Dnp)-OH. |
| MW | ~120kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <0.1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS. |
| Other Product Data | Swiss-Prot link Q9NRA7: Angiotensin converting enzyme-like protein (human) |
| Product Type | Protein |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
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Product Description
ACE2 has direct effects on cardiac functions. It is expressed predominantly in vascular endothelial cells of the heart and kidney. ACE2 converts angiotensin I to angiotensin 1-9. ACE2 was also identified as a functional receptor for SARS coronavirus.
Product References
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Hydrolysis of a fluorescent peptide substrate, Mca-Y-V-A-D-A-P-K(Dnp)-OH.
To each test tube, 2ml of assay buffer (75mM Tris, pH 7.4, 1M NaCl, 10 μM zinc chloride, 10 μM fluorescent peptide substrate) was added. Reactions were initiated by the addition of 20 μl (1 μg/ml, final 10ng/ml) of human ACE2-Fc (Prod. No. A-40A-0026), Fc fusion control protein, and buffer only (for blank). Reactions were performed for 20min at room temperature and stopped by the addition of 0.2ml 500mM EDTA. The fluorescence intensity was measured using luminescence spectrometer (excitation = 320nm, emission = 405nm).
To each test tube, 2ml of assay buffer (75mM Tris, pH 7.4, 1M NaCl, 10 μM zinc chloride, 10 μM fluorescent peptide substrate) was added. Reactions were initiated by the addition of 20 μl (1 μg/ml, final 10ng/ml) of human ACE2-Fc (Prod. No. A-40A-0026), Fc fusion control protein, and buffer only (for blank). Reactions were performed for 20min at room temperature and stopped by the addition of 0.2ml 500mM EDTA. The fluorescence intensity was measured using luminescence spectrometer (excitation = 320nm, emission = 405nm).
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