Additional Information
| Product Data | |
|---|---|
| Synonyms | ACRP30; AdipoQ; apM1; GBP28; Adipocyte Complement Related Protein of 30kDa |
| Properties | |
| Source/Host | E. coli |
| Sequence | The globular domain of human adiponectin (aa 104-244) is fused at the C-terminus to a His-tag. |
| Crossreactivity | Human |
| Biological Activity | Induces the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells. |
| MW | ~17kDa (SDS-PAGE) |
| Purity | ≥95% (SDS-PAGE) |
| Endotoxin Content | <1EU/μg purified protein (LAL test; Lonza). |
| Concentration | 1mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in 30mM TRIS-Cl, pH 8.5. |
| Other Product Data | Uniprot link Q15848: Adiponectin (human) (precursor) |
| Product Type | Protein |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Working aliquots are stable for up to 3 months when stored at -20°C. |
| Documents | |
| MSDS |
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Product Description
ACRP30 was identified as a novel adipocyte-specific synthesized and secreted protein with structural resemblance to complement factor C1q. Like adipsin, ACRP30 secretion is induced ~10-fold during adipocyte differentiation. Plasma levels are reduced in obese humans and low levels are associated with insulin-resistance. Treatment of db/db mice with TZD increased ACRP30 levels.
Effect of Adiponectin (mouse) (rec.) (Prod. No. AG-40A-0005) on the phosphorylation of acetyl-CoA carboxylase (ACC) in C2C12 cells.
C2C12 myocytes were treated with 3μg/ml for 15min after serum starvation for 24hr. 100μg per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.
1: 3μg/ml of Adiponectin (globular domain) (human) (rec.) (His).
2: 3μg/ml of a His-tagged control protein.
3: phosphate-buffered saline.
C2C12 myocytes were treated with 3μg/ml for 15min after serum starvation for 24hr. 100μg per well of total cell lysate was separated on reduced SDS-PAGE gels. Immunoblot analysis was performed to measure phosphorylation of ACC with an anti-phosphoACC antibody.
1: 3μg/ml of Adiponectin (globular domain) (human) (rec.) (His).
2: 3μg/ml of a His-tagged control protein.
3: phosphate-buffered saline.
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