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anti-LRP5/6, mAb (rec.) (Heldy-1-4)

AG-27B-0019-C100 100 µg INQ
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Additional Information

Product Data
Synonyms Low-density Lipoprotein Receptor-related Protein 5; LRP-5; Low-density Lipoprotein Receptor-related Protein 6; LRP-6
Properties
Clone Heldy-1-4
Isotype Human IgG2λ.
Source/Host HEK 293 cells
Immunogen/Antigen Recombinant mouse LRP6.
Application Flow Cytometry: 1/100 dilution
Crossreactivity Human, Mouse
Specificity Recognizes human and mouse LRP5 & LRP6.
Purity ≥95% (SDS-PAGE)
Purity Detail Protein A-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol.
Other Product Data anti-LRP5/6 (human), mAb (rec.) (Heldy-1-4) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain.
Product Type Recombinant Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
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Product Description

Wnt/β-catenin signaling acts as a regulator of tissue development and homeostasis. Wnt ligands bind to a member of the Frizzled family of seven-transmembrane proteins and to either highly homologous low-density lipoprotein related receptors 5 (LRP5) or LRP6. Low-density lipoprotein receptor-related protein 6 (LRP6) is a member of the low-density lipoprotein receptor (LDLR) family. LRP5 and LRP6 are expressed in most human tissues and is composed of three distinctive cell-surface protein domains, including (1) LDL receptor (LDLR) type A repeats; (2) an epidermal growth factor (EGF)-like domain; and (3) a YWTD (Tyr-Trp-Thr-Asp)-type β propeller domain. LRP6 is inhibited by the Dickkopf protein Dkk1 bound to their partners Kremen. Sclerotin also acts as an inhibitor of LRP5/6 through LRP4. LRP6 is involved in cell differentiation, proliferation and migration during embryonic development and in the pathogenesis of different cancer types. LRP6 plays a major role in metabolic regulation, specifically in the nutrient-sensing pathway. Patients carrying an LRP6 mutation exhibit high serum LDL cholesterol and triglycerides, hypertension, high fasting blood glucose levels, a prevalence of diabetes and low bone density. LRP6 mutations have also been associated with diseases such as Alzheimer's and degenerative joint diseases. LRP5, low-density-lipoprotein-receptor-related protein 5, is a determinant for bone mineral density and plays a role in osteoporosis. LRP5 is aso involved in vitreoretinopathy.
Detection of endogenous mouse LRP5/6 using anti-LRP5/6, mAb (rec.) (Heldy-1-4) (Prod. No. AG-27B-0019). <strong>Method:</strong> B16 melanoma mouse cells were stained with anti-LRP5/6, mAb (rec.) (Heldy-1-4) (blue and red lines) (1/100 in PBS + 2% FCS) or with the secondary antibody alone as control (gray area) for 1h at 4&deg;C. The staining was revealed with a secondary anti-human IgG-PE (1/200) and then analyzed by flow cytometry. The blue and red lines represent two different lots of anti-LRP5/6, mAb (rec.) (Heldy-1-4).  <em>Picture courtesy of Prof. Werner Held lab, Ludwig Institute for Cancer Research, Lausanne, Switzerland.</em>
Detection of endogenous mouse LRP5/6 using anti-LRP5/6, mAb (rec.) (Heldy-1-4) (Prod. No. AG-27B-0019). Method: B16 melanoma mouse cells were stained with anti-LRP5/6, mAb (rec.) (Heldy-1-4) (blue and red lines) (1/100 in PBS + 2% FCS) or with the secondary antibody alone as control (gray area) for 1h at 4°C. The staining was revealed with a secondary anti-human IgG-PE (1/200) and then analyzed by flow cytometry. The blue and red lines represent two different lots of anti-LRP5/6, mAb (rec.) (Heldy-1-4). Picture courtesy of Prof. Werner Held lab, Ludwig Institute for Cancer Research, Lausanne, Switzerland.
Detection of overexpressed human LRP6 using anti-LRP5/6, mAb (rec.) (Heldy-1-4) (Prod. No. AG-27B-0019). <strong>Method:</strong> HEK293 cells transfected (green line) or mock transfected (black line) with a dicistronic plasmid encoding for human LRP6 and GFP, were stained with anti-LRP5/6, mAb (rec.) (Heldy-1-4) (1/100 in PBS + 2% FCS) or with the secondary antibody alone as control (gray area) for 1h at 4&deg;C. The staining was revealed with an anti-human IgG PE (1/200) and then analyzed by flow cytometry.  <i>Picture courtesy of Prof. Werner Held lab, Ludwig Institute for Cancer Research, Lausanne, Switzerland.</i>
Detection of overexpressed human LRP6 using anti-LRP5/6, mAb (rec.) (Heldy-1-4) (Prod. No. AG-27B-0019). Method: HEK293 cells transfected (green line) or mock transfected (black line) with a dicistronic plasmid encoding for human LRP6 and GFP, were stained with anti-LRP5/6, mAb (rec.) (Heldy-1-4) (1/100 in PBS + 2% FCS) or with the secondary antibody alone as control (gray area) for 1h at 4°C. The staining was revealed with an anti-human IgG PE (1/200) and then analyzed by flow cytometry. Picture courtesy of Prof. Werner Held lab, Ludwig Institute for Cancer Research, Lausanne, Switzerland.
Structure of the recombinant antibody anti-LRP5/6, mAb (rec.) (Heldy-1-4) (Prod. No. AG-27B-0019). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.
Structure of the recombinant antibody anti-LRP5/6, mAb (rec.) (Heldy-1-4) (Prod. No. AG-27B-0019). The single chain variable human fragment (ScFv) selected by antibody phage display technology and specific to the antigen of interest is fused to a human IgG2 Fc region.
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