Additional Information
| Product Data | |
|---|---|
| Synonyms | Cellular Myosin Heavy Chain, Type A; Myosin Heavy Chain 9; Myosin Heavy Chain, Non-muscle IIa; Non-muscle Myosin Heavy Chain A |
| Properties | |
| Clone | SF9 |
| Isotype | Human IgG2λ |
| Source/Host | Purified from HEK 293 cell culture supernatant. |
| Immunogen/Antigen | Full length myosin IIA from rat liver. |
| Application | Electron Microscopy ELISA Immunocytochemistry: (1:1000) Western blot: (1:1000) |
| Crossreactivity | Drosophila, Human, Mouse, Rat |
| Specificity | Recognizes human, mouse, rat and drosophila myosin IIA (heavy chain). |
| Purity | ≥95% (SDS-PAGE) |
| Purity Detail | Protein A-affinity purified. |
| Concentration | 1mg/ml |
| Formulation | Liquid. In PBS containing 10% glycerol and 0.02% sodium azide. |
| Other Product Data | anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is composed of human variable regions (VH and VL) (λ-chain) of immunoglobulin fused to the human lgG2 Fc domain. |
| Product Type | Recombinant Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Use/Stability | Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C. |
| Documents | |
| MSDS |
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Product Description
anti-Myosin IIA (non-muscle), monoclonal antibody (recombinant) (SF9) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
Product References
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Schematic antibody structure.
Human Myosin IIA (non-muscle) (heavy chain) is detected by immunocytochemistry using anti-myosin IIA (non-muscle) (rec.) (SF9) (Prod. No. AG-27B-0010).
Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-Myosin IIA (non-muscle) (rec.) (SF9) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.
Method: HeLa cells are grown in standard culture conditions, fixed with methanol, and incubated with anti-Myosin IIA (non-muscle) (rec.) (SF9) (1µg /ml in PBS-BSA). After incubation for 30 min at RT and several washes in PBS, cells are treated with a goat anti-human (Cy3) antibody for 30 min at RT, washed and mounted in Moewiol. Nuclei are stained with DAPI.
Picture courtesy of Dr. Moutel, Dr. Franck Perez lab, Curie Institute, Paris.
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