|Synonyms||OB Gene Product; Obese Factor|
|Immunogen/Antigen||Recombinant mouse leptin.|
|Application||ELISA: (direct and indirect: 1:5’000-1:10’000)
Immunoprecipitation: (1-5 μl per sample in a final reaction volume of 0.5-1 ml)
Western Blot: (1:5’000-1:10’000 using ECL. Suggested blocking and dilution buffer is TBST containing 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at room temperature).
Optimal conditions must be determined individually for each application.
|Specificity||Recognizes mouse leptin. Weakly cross-reacts with human and rat leptin. Detects a band of ~16kDa by Western blot.|
|Purity Detail||Protein A-affinity purified.|
|Formulation||Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.|
|Product Type||Polyclonal Antibody|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.|
Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.
Western blot analysis of recombinant leptin (His-tagged) and leptin Fc-protein using anti-Leptin (mouse), pAb (Biotin) (Prod. No. AG-25A-0008B) at 1:5'000 dilution.
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