AdipoGen Life Sciences

anti-NAIP1/2/5 (mouse), mAb (Naipa-1)

CHF 480.00
In stock
AG-20B-0045-C100100 µgCHF 480.00
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Product Details
Synonyms Neuronal Apoptosis Inhibitory Protein 1, 2, 5; Baculoviral IAP Repeat-containing Protein 1a, 1b, 1e; Birc 1a, 1b, 1e
Product Type Monoclonal Antibody
Properties
Clone Naipa-1
Isotype Mouse IgG2bκ
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant mouse NAIP5 (BIR2 domain/aa 159-227).
Application

Western Blot: (1μg/ml)

Crossreactivity Mouse
Specificity

Recognizes mouse NAIP1, 2 and 5. Not tested on mouse NAIP6 and mouse NAIP7 but should also work due to high homology in the BIR2 domain.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Mouse IgG2b Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

The inflammasome is a multiprotein complex that mediates the activation of caspase-1, which promotes, amongst others, the secretion of the proinflammatory cytokines interleukin (IL)-1β and IL-18. Members of the Nod-like receptor (NLR) family are critical components of the inflammasome that link danger-signals to caspase-1 activation. The NLR member IPAF/NLRC4 senses bacterial flagellin and a conserved type III secretion system (TTSS) rod component. A member of the NLR family, NAIP5 directly and specifically interacts with flagellin, triggers a physical NAIP5-NLRC4 association leading to NLRC4 activation. The related NAIP2 functions analogously to NAIP5, serving as a specific inflammasome receptor for TTSS rod proteins such as Salmonella PrgJ and Burkholderia BsaK.

Product References
  1. CD137 ligand-CD137 interaction is required for inflammasome-associated brain injury following ischemic stroke: D.Y. Fann, et al.; NeuroMol. Med. 22, 474 (2020)
  2. Kinetics of free and ligand-bound atacicept in human serum: M. Eslami, et al.; Front. Immunol. 13, 1035556 (2022)
  3. Inflammasome Activation Mediates Apoptotic and Pyroptotic Death in Astrocytes Under Ischemic Conditions: LJ. Wong, et al.; Neuromolecular Med. ahead of print (2023)
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