Neutrophils (mouse), mAb antibody (Nimp-R14) (Blocking)

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Properties
Clone	Nimp-R14
Isotype	Rat IgG2b
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Purified mouse BALB/c neutrophils.
Application	Flow Cytometry:(1:100) Immunohistochemistry:(1:200; frozen section / paraffin section) Immunocytochemistry: (1:200) Functional (Blocking): 250μg / mouse (Optimal reagent to deplete neutrophils in vivo)
Crossreactivity	Mouse
Specificity	Recognizes mouse neutrophils (surface protein mouse Ly-6G).
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Endotoxin Content	<0.1EU/μg purified protein (LAL test; Lonza).
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Product Type	Monoclonal Antibody
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
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Product Description

Neutrophils are key components of the inflammatory response and as such contribute to the killing of microorganisms. In addition, recent evidence suggests their involvement in the development of the immune response. The Nimp-R14 mAb displays features of an optimal reagent allowing the selective depletion of mouse neutrophils in vivo.

Product References

Differentiation antigens on mouse eosinophils and neutrophils identified by monoclonal antibodies: A. Lopez, et al.; Brit. J. Haematol. 57, 489 (1984) Schistosoma mansoni: evidence that immunity in vaccinated and chronically infected CBA/Ca mice is sensitive to treatment with a monoclonal antibody that depletes cutaneous effector cells: D. McLaren, et al.; Parasite Immunol. 9, 667 (1987) Monocytes/macrophages rather than PMN are involved in early cartilage degradation in cationic immune complex arthritits in mice: P. Van Lent, et al.; J. Leukoc. Biol. 61, 267 (1997) Adenoviral vector-mediated overexpression of IL-4 in the knee joint of mice with collagen-induced arthritis prevents cartilage destruction: E. Lubberts, et al.; J. Immunol. 163, 4546 (1999) An immunomodulatory function for neutrophils during the induction of a CD4+ Th2 response in BALB/c mice infected with Leishmania major: F. Tacchini-Cottier, et al.; J. Immunol. 165, 628 (2000) A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5: K.M. Al-Qaoud, et al.; Int. Immunol. 12, 899 (2000) Rapid recruitment of inflammatory monocytes is independent of neutrophil migration: R.B. Henderson, et al.; Blood 102, 328 (2003) Complement factor C5a mediates renal ischemia-reperfusion injury independent from neutrophils: B. de Vries, et al.; J. Immunol. 170, 3883 (2003) Absence of cross-reactivity between murine Ly-6C and Ly-6G: S. Nagendra, et al.; Cytom. A 58, 195 (2004) Activation of Toll-Like Receptor (TLR)2, TLR4, and TLR9 in the Mammalian Cornea Induces MyD88-Dependent Corneal Inflammation: A.C. Johnson, et al.; Invest. Ophthalmol. Vis. Sci. 46, 589 (2005) Neutrophils contribute to development of a protective immune response during onset of infection with Leishmania donovani: E. McFarlane, et al.; Infect. Immun. 76, 532 (2008) Innate Immune-induced depletion of bone marrow neutrophils aggravates systemic bacterial infections: A. Navarini, et al.; PNAS 106, 7107 (2009) Analysis of innate defences against Plasmodium falciparum in immunodeficient mice: A Ludovic, et al.; Malaria J. 9, 197 (2010) Role of Neutrophils in the Early Shaping of the Leishmania Major Specific Immune Response in Experimental Murine Cutaneous Leishmaniasis: M. Charmoy, et al.; Neutrophils in Infectious Diseases 1, 49 (2011)

Mouse neutrophils are depleted <i>in vivo</i> by anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (AG-20B-0043). <br /> <b>Method:</b> Mice were injected) i.p. with 250 µg of anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (B) or with Control mAb (A) in BALB/c mouse 6 h prior to <i>Leishmania major</i> infection (3x10<sup>6</sup> parasites injected in the hind footpad). 3 days later, blood (100µl) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).

Mouse neutrophils are depleted in vivo by anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (AG-20B-0043).
Method: Mice were injected) i.p. with 250 µg of anti-Neutrophils (mouse), mAb (blocking) (Nimp-R14) (B) or with Control mAb (A) in BALB/c mouse 6 h prior to Leishmania major infection (3x10⁶ parasites injected in the hind footpad). 3 days later, blood (100µl) was subjected to flow cytometric analysis after staining with APC/CY7-labeled anti-Ly6G antibody (clone 1A8).

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