anti-MDA5 (human), mAb antibody (Hely-1)

Specifications / Handling

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Product Details
Synonyms	Helicard; Melanoma Differentiation-associated Gene 5; Interferon-induced helicase C domain-containing protein 1; IFIH1
Product Type	Monoclonal Antibody
Properties
Clone	Hely-1
Isotype	Mouse IgG1
Source/Host	Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen	Recombinant human MDA5 (aa 78-555).
Application	ELISA Immunoprecipitation: (1:100) Western Blot: (1:1‘000)
Crossreactivity	Human
Specificity	Recognizes human MDA5. Detects a band at ~115-120kDa by Western blot.
Purity	≥95% (SDS-PAGE)
Purity Detail	Protein G-affinity purified.
Concentration	1mg/ml
Formulation	Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control	Mouse IgG1 Isotype Control
Accession Number	Q9BYX4
Shipping and Handling
Shipping	BLUE ICE
Short Term Storage	+4°C
Long Term Storage	-20°C
Handling Advice	After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
Use/Stability	Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS	Download PDF
Product Specification Sheet
Datasheet	Download PDF

Product-specific References

Description

RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-1 recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.

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anti-MDA5 (human), mAb (Hely-1)