AdipoGen Life Sciences

anti-RIG-I, mAb (Alme-1)

CHF 495.00
In stock
AG-20B-0009-C100100 µgCHF 495.00
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Product Details
Synonyms RIG-1; Retinoic Acid-inducible Gene 1 Protein; DEAD-box Protein 58; Probable ATP-dependent RNA Helicase DDX58
Product Type Monoclonal Antibody
Properties
Clone Alme-1
Isotype Mouse IgG1
Source/Host Purified from concentrated hybridoma tissue culture supernatant.
Immunogen/Antigen Recombinant human RIG-I (aa 201-713).
Application

Immunohistochemistry: (paraffin sections)
Immunoprecipitation: (1:200)
Western blot: (1:1’000)

Crossreactivity Human
Mouse
Specificity

Recognizes human and mouse RIG-I.

Purity ≥95% (SDS-PAGE)
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. In PBS containing 10% glycerol and 0.02% sodium azide.
Isotype Negative Control

Mouse IgG1 Isotype Control

Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After opening, prepare aliquots and store at -20°C.
Avoid freeze/thaw cycles.
Use/Stability Stable for at least 1 year after receipt when stored at -20°C.
Documents
MSDS Download PDF
Product Specification Sheet
Datasheet Download PDF
Description

RIG-I and MDA5 are highly conserved helicases involved in the innate immune response to virus. RIG-I is a member of the DEAD-box RNA helicases and is activated by cytoplasmic dsRNA and 5’-ppp RNA produced during the viral replication. The protein is characterized by a N-terminal region with two caspase recruitment domains (CARD) and a C-terminal region harboring potential ATP-dependent RNA helicase activity. RIG-I recruits the CARD adaptor inducing IFN-β (Cardif) in a CARD-CARD-dependent manner resulting in NF-κB and IRF3 activation.

Product References
  1. The antiviral adaptor proteins Cardif and Trif are processed and inactivated by caspases: M. Rebsamen, et al.; Cell Death Differ. 15, 1804 (2008)
  2. Phosphorylation-mediated negative regulation of RIG-I antiviral activity: M.U. Gack, et al.; J. Virol. 84, 3220 (2010)
  3. Incoming RNA Virus Nucleocapsids Containing a 5'-Triphosphorylated Genome Activate RIG-I and Antiviral Signaling: M. Weber, et al.; Cell Host Microbe 13, 336 (2013)
  4. Lymphocytic choriomeningitis virus differentially affects the virus-induced type I interferon response and mitochondrial apoptosis mediated by RIG-I/MAVS: C. Pythoud, et al.; J. Virol. 89, 6240 (2015)
  5. A phosphomimetic-based mechanism of dengue virus to antagonize innate immunity: Y.K. Chan & M.U. Gack; Nat. Immunol. 17, 523 (2016)
  6. Sensing of latent EBV infection through exosomal transfer of 5'pppRNA: S.R. Baglio, et al.; PNAS 113, E587 (2016)
  7. RNAs Containing Modified Nucleotides Fail To Trigger RIG-I Conformational Changes for Innate Immune Signaling: A. Fiegen Durbin, et al.; MBio 7, e00833 (2016)
  8. Viral unmasking of cellular 5S rRNA pseudogene transcripts induces RIG-I-mediated immunity: J.J. Chiang, et al.; Nat. Immunol. 19, 53 (2018)
  9. RIG-I recognizes the 5′ region of dengue and zika virus genomes: M. Chazal, et al.; Cell Rep. 24, 320 (2018)
  10. The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling: C. Chiang, et al.; J. Virol. 92, e01737-17 (2018) [KO Validation]
  11. Zika virus NS3 mimics a cellular 14-3-3-binding motif to antagonize RIG-I- and MDA5-mediated innate immunity: W. Riedl, et al.; Cell Host Microbe 26, 493 (2019)
  12. Influenza A virus M2 protein triggers mitochondrial DNA-mediated antiviral immune responses: M. Moriyama, et al.; Nat. Comm. 10, 6424 (2019)
  13. Attenuation of the Innate Immune Response against Viral Infection Due to ZNF598-Promoted Binding of FAT10 to RIG-I: G. Wang, et al.; Cell Rep. 28, 1961 (2019)
  14. ISG15-dependent activation of the sensor MDA5 is antagonized by the SARS-CoV-2 papain-like protease to evade host innate immunity: GQ. Liu, et al.; Nat. Microbiol. 6, 465 (2021)
  15. NSs of the mildly virulent sandfly fever Sicilian virus is unable to inhibit interferon signaling and upregulation of interferon-stimulated genes: J.D. Wuerth & F. Weber; J. Gen. Virol. 102, (2021)
  16. Viral Protein Accumulation of Zika Virus Variants Links with Regulation of Innate Immunity for Differential Control of Viral Replication, Spread, and Response to Interferon: A.Y. Lu, et al.; J. Virol. 97, e0198222 (2023)
  17. Acetylation of the NS3 helicase by KAT5g is essential for flavivirus replication: T. Serman, et al.; Cell Host Microbe 31, 1317 (2023)
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