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anti-Leptin (human), mAb (HLEP 155)

AG-20A-0019-C050 50 µg $170.00
AG-20A-0019-C100 100 µg $240.00
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Additional Information

Product Data
Synonyms OB Gene Product; Obese Factor
Properties
Clone HLEP 155
Isotype Mouse IgG1κ
Immunogen/Antigen Recombinant human leptin.
Application ELISA: (direct or indirect: 1:5'000-1:10'000)
Western blot: (1:2'000-1:10'000 using ECL).
Optimal conditions should be determined individually for each application.
Crossreactivity Human
Specificity Recognizes human leptin. Detects a band of ~16kDa by Western blot. Does not cross-react with mouse or rat leptin.
Purity Detail Protein G-affinity purified.
Concentration 1mg/ml
Formulation Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.
Product Type Monoclonal Antibody
Shipping and Handling
Shipping BLUE ICE
Short Term Storage +4°C
Long Term Storage -20°C
Handling Advice After receipt, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.
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Product Description

Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.
Western Blot analysis using anti-Leptin (human), mAb (HLEP 155) (Prod. No. AG-20A-0019) at 1:5000 dilution.<br />
1: Recombinant human Leptin protein. <br />
2: Human Leptin Fc-fusion protein.
Western Blot analysis using anti-Leptin (human), mAb (HLEP 155) (Prod. No. AG-20A-0019) at 1:5000 dilution.
1: Recombinant human Leptin protein.
2: Human Leptin Fc-fusion protein.
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