Additional Information
| Product Data | |
|---|---|
| Synonyms | FIZZ3; Found in Inflammatory Zone 3; ADSF; Adipose Tissue-Specific Secretory Factor |
| Properties | |
| Clone | RRES 03 |
| Isotype | Mouse IgG2aκ |
| Immunogen/Antigen | Recombinant rat resistin. |
| Application | ELISA: (direct or indirect: 1:5'000-1:10'000) Western blot: (1:2'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature). Optimal conditions should be determined individually for each application. |
| Crossreactivity | Rat |
| Specificity | Recognizes rat resistin. Detects a band of ~10kDa by Western blot. Weakly cross-reacts with human and mouse resistin. |
| Purity Detail | Protein G-affinity purified. |
| Concentration | 0.5mg/ml |
| Formulation | Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives. |
| Product Type | Monoclonal Antibody |
| Shipping and Handling | |
| Shipping | BLUE ICE |
| Short Term Storage | +4°C |
| Long Term Storage | -20°C |
| Handling Advice | After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles. |
| Documents | |
| MSDS | 
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Product Description
The adipocytokine resistin which belongs to a family of cysteine-rich C-terminal proteins known as resistin-like molecules (RELM; RELMα/FIZZ 1 and RELMβ/FIZZ 2) of FIZZ (found in inflammatory zone) are thought to be involved in inflammatory processes. Previous studies in mice showed that resistin impairs glucose tolerance and insulin action. In addition, resistin also inhibits adipogenesis in murine 3T3-L1 cells. Therefore resistin has also been proposed as an adipocyte-secreted factor thought to link obesity and T2DM.
Western blot analysis of resistin using anti-Resistin (rat), mAb (RRES 03) (Prod. No. AG-20A-0014) at 1:5'000 dilution.
1: Rat resistin:Fc fusion protein.
2: Recombinant rat resistin protein (His-tagged).
3: Recombinant human resistin protein (His-tagged).
4: Recombinant mouse resistin protein (His-tagged).
1: Rat resistin:Fc fusion protein.
2: Recombinant rat resistin protein (His-tagged).
3: Recombinant human resistin protein (His-tagged).
4: Recombinant mouse resistin protein (His-tagged).
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