|Synonyms||OB Gene Product; Obese Factor|
|Immunogen/Antigen||Recombinant human leptin.|
|Application||ELISA: (direct or indirect: 1:5'000-1:10'000)
Western blot: (1:5'000-1:10'000 using ECL. Suggested blocking and dilution buffer is TBST with 0.05% Tween 20 and 5% skim milk. Suggested incubation time is 1 hour at rooom temperature).
Optimal conditions should be determined individually for each application.
|Specificity||Recognizes human leptin. Detects a band of ~25kDa by Western blot.|
|Purity Detail||Protein G-affinity purified.|
|Formulation||Liquid. 0.2μm-filtered solution in PBS, pH 7.4. Contains no preservatives.|
|Product Type||Monoclonal Antibody|
|Shipping and Handling|
|Short Term Storage||+4°C|
|Long Term Storage||-20°C|
|Handling Advice||After opening, prepare aliquots and store at -20°C. Avoid freeze/thaw cycles.|
Leptin is involved in the signaling pathway that regulates the size of the body fat depot. An increase in the level of leptin may act directly or indirectly on the CNS to inhibit food intake and/or regulate energy expenditure as part of a homeostatic mechanism to maintain constancy of the adipose mass. Defects in the leptin gene may be a cause of autosomal recessive obesity.
Western Blot analysis of recombinant leptin using anti-Leptin (human), mAb (HLEP 55) (Prod. No. AG-20A-0011) at 1:5000 dilution.
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