AdipoGen offers a unique test for measuring the inflammasome activity of extracellular signals. Features of the test are:
• Testing nanoparticles or other chemicals (samples) on inflammasome activity using human macrophages THP1 WT and THP1 NLRP3/NALP3 KO.
• Measurement of secreted human IL-1β using a specific IL-1β (human) ELISA Kit.
• Performing test at 4 different concentrations of samples.
• Use of positive and negative controls.
• Samples are tested on endotoxin content.
• Final report in 2-3 weeks upon receipt of samples.
Literature Reference:
Nanoparticles activate the NLR pyrin domain containing 3 (Nlrp3) inflammasome and cause pulmonary inflammation through release of IL-1α and IL-1β: A.S. Yazdi, et al.; PNAS 107, 19449 (2010)
Typical Result: Effect of Sample on human IL-1β secretion: comparison to positive control (titanium oxide; TiO2) and negative control (zinc oxide; ZnO)
Figure: Measurement of IL-1β upon treatment of THP1 cells at different sample concentrations.
Method: THP1 cells were incubated with indicated concentrations of TiO2, ZnO and Sample for 6 hours. Secreted IL-1β was measured using a specific IL-1β (human) ELISA Kit.
What does the Flammasome™ Test detect?
Interleukin-1β (IL-1β) is a potent mediator of inflammation. Pro-IL-1β is cleaved by the protease caspase-1 that itself is autoactivated by the large multiprotein complexes called inflammasomes (e.g. NLRP3/NALP3). Following cleavage of the pro-IL-1β, the biologically active form IL-1β is secreted, which is detected by the Flammasome™ Test. The signal required for the inflammasome formation can be of heterogeneous nature, including particulate stimuli, pathogens, pore-forming toxins or extracellular ATP.
